孕大鼠在胚泡着床前给予醋氨酚对器官形成期胚胎发育的影响

Effects of Acetaminophen Administration in Preimplantation on Embryo Development During Organogenesis of Rats

研究孕大鼠胚泡着床前药物损伤对胚胎器官形成期发育的影响，探索药源性胚泡异常与胎仔发育的关系。方法：母鼠孕第3天给予醋氨酚0．25～1．00g／kg．孕第4天处死母鼠．计数胚泡细胞数，胚泡微核数及微核率；孕第12．5天处死母鼠，评价胚胎组织器官形态分化程度及胚胎生长发育状况。结果：醋氨酚（0．25～1．00g／kg）抑制着床前胚泡发育,对胚泡细胞产生遗传毒作用，并呈明显剂量依赖关系。与对照组相比，孕第12．5天（器官形成期）胚胎生长发育状况无明显差异，胚胎组织器官形态分化无显著改变。醋氨酚0．5g，1．0g／kg对胚胎发育具毒性，导致胚胎发育迟缓、胚胎畸形、胚胎死亡数显著增加。结论：胚胎发育毒性与着床前胚泡遗传物质损伤相关。

ObjectiveTo evaluate the effects of blastocysts damaged by drug on the development of embryosduring organogenesis. Methods: Acetaminophen (Ace) was administrated to pregnant rats on day 3 of gestation(Positive vaginal smear= day 0). The cell number of blastocysts, frequency of blastocysts with micronuclei(FM) and frequency of blastocysts with micronuclei Per blastocyst (FBM) were counted. The growth and morphology indices of embryos on day 12. 5 of gestation were evaluated. Results: Administrated of Ace 0. 5. 1. 0g / kg to pregnant rats on day 3 of gestation induced genetic toxicity in blastocysts. There was a dose - relatedincrease in FM and FBM. There was no significant difference between Ace 0. 5, 1. 0 g/kg and control group ingrowth and morphology indices. A marked increase of embryos with developmental toxicity was recorded ineither group treated with Ace. Conclusion: Ace caused significant increase of abnormal embryos related to thedamage of genetic material of blastocysts.