Notch信号通路相关基因的表达与涎腺腺样囊性癌转移的关系

Gene Expression Profile of Notch Signaling Pathway and the Metastasis of Adenoid Cystic Carcinoma

目的筛选与涎腺腺样囊性癌转移相关的Notch信号通路基因。方法对已构建的涎腺腺样囊性癌高低转移细胞株ACC-M、ACC-2基因表达谱进行生物信息学分析,在与Notch信号通路基因进行比对后,筛选出差异基因,通过荧光实时定量PCR技术对相关基因的表达差异进行初步验证。结果涎腺腺样囊性癌的表达谱中有46个基因与Notch信号通路有关。验证发现,Notch-1,Notch-2,Notch-4,CHUK,FOXC1,FZD2,FZD3,GBP2和RUNX1在2个细胞株中的表达差别有统计学意义(P<0.05),同时Notch-1在发生转移的涎腺腺样囊性癌中的表达明显高于未发生转移的涎腺腺样囊性癌(P<0.05)。结论Notch信号通路中的多个基因可能参与了涎腺腺样囊性癌的发生和发展;Notch-1的表达与涎腺腺样囊性癌的发生、转移密切相关。

tastasis of adenoid Objective To screen genes of Notch signaling pathway, which are related to the mecystic carcinoma. Methods The gene expression profile of adenoid cystic carcinoma cell lines with high and low metastasis potential, ACC-M and ACC-2, were conducted with bioinformatics analysis. By comparison with genes of Notch signaling pathway, differentially expressed genes were identified. Real time-PCR was Used to examine the mRNA level of these genes in ACC-2 and ACC-M. Results 46 genes in the expression profile of adenoid cystic carcinoma were correlated to Notch signaling pathway. With Real time-PCR, it was demonstrated that the expressions of Notch-1, Notch-2, Notch-4, CHUK, FOXC1, FZD2, FZD3, GBP2 and RUNX-1 in ACC-M were significantly higher as compared with those in ACC-2 （P（0.05）. The expression of Notch-1 was significantly upregulated in adenoid cystic carcinoma as compared with that in normal tissues or benign tumor, and was higher in adenoid cystic carcinoma with metastasis than that in the case without metastasis. Conclution It is shown that genes of Notch signaling pathway,especially Notch-1, may play a certain role in tumorigenesis, progression, invasion and metastasis of salivary adenoid cystic carcinoma.