Let-7c在乳腺浸润性导管癌中的表达及临床意义

Expression of Let-7c in invasive ductal carcinoma of breast cancer and its clinical significance

目的:探讨Let-7c在乳腺浸润性导管癌中的表达情况及与各种临床病理特征的关系。方法:采用茎环RT-PCR方法检测40例乳腺浸润性导管癌及其对应的正常乳腺组织中Let-7c的表达,分析Let-7c表达与临床分期、淋巴结转移、增殖指数等临床病理特征的关系。结果:用茎环RT-PCR方法检测Let-7c表达的敏感性和特异性良好;Let-7c在乳腺癌组织中的表达显著低于正常组织(P<0.05);Let-7c的表达与乳腺癌的增殖指数有关(P<0.05),与雌孕激素受体状态亦有关,与是否绝经、乳腺癌的TNM分期、淋巴结转移未见显著统计学意义(P>0.05)。在高增殖指数、雌孕激素受体阴性的肿瘤组Let-7c表达明显降低。结论:Let-7c在乳腺浸润性导管癌中的表达异常,可能和乳腺癌的发生和发展有关,有望成为乳腺癌新的预后指标及治疗靶点。

Objective：To investigate the expression of Let-7c in patients with invasive ductal carcinoma of the breast cancer and its correlation with the clinicopathologic features of the patients. Methods： The expression of Let 7c was examined in 40 tissues of invasive ductal carcinoma of the breast cancer and their matched non-tumor adjacent tissue specimens by stem-loop real-time RT-PCR. The correlation of the expression with the clinicopathological features of breast cancer, such as the clinical staging, lymphatic metastasis, and proliferation index, was analyzed. Results： The stern loop realtime RT-PCR was sensitive and specific in detecting Let-7c expression. Expression of Let-7c in breast cancer tissues was significantly lower than that in the adjacent normal tissues（P〈0. 05）. Downregulation of Let-7c expression was associated with higher proliferation index （Ki-67〉 10 %） （P=0. 030） and the status of Estrogen/ Progesterone receptor （P= 0. 040/0. 034）in breast cancer patients. There was no significant association of Let-Tc expression with menopause condition, breast cancer TNM clinical stage, or lymphatic metastasis （ P〉0. 05）. Conclusion： Our results suggest Let-7c may play an important role in the development of breast cancer and the level of Let-7c expression may be a potential parameter in forecasting the progression and prognosis of breast cancer patients.