微生物来源的免疫抑制活性物质 Ⅰ.抗生素SIPI-84-15的研究

Immunosuppressive compounds from microorganisms Ⅰ. Studies on antibiotic SIPI8415

用酵母系统筛选微生物产生的免疫抑制活性化合物，从３００多株放线菌中筛选出阳性菌株ＳＩＰＩ－９７－８４，对其代谢产物进行初步研究，用丙酮浸泡、溶媒萃取、硅胶柱层析和重结晶等方法，从其菌丝体中分离出活性成分ＳＩＰＩ－８４－１５。通过元素分析和质谱，确定其分子式为Ｃ２８Ｈ３１ＮＯ６（分子量：４７７．２１４５）；综合紫外光谱、红外光谱、１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ等图谱数据，确定ＳＩＰＩ－８４－１５的结构为含有硝基的多烯化合物，该结构与文献报道的ｓｐｅｃｔｉｎａｂｉｌｉｎ一致，化学命名为：２－甲氧基－３，５－二甲基－６－［四氢－４－［２，４，６－三甲基－７－（４－硝基苯基）－２，４，６－庚三烯亚基］－２－呋喃基］，４Ｈ－吡喃－４－酮。

Using a method for screening immunosuppressive compounds from microorganisms in a yeast system,a positive strain named SIPI9784 whose metabolites exhibited inhibitory activities on yeasts was obtained from more than 300 Actinomycetes collected in our laboratory.Physiologically active compound SIPI8415 was isolated from the mycelia of Streptomyces sp. 9784 by acetone soaking, solvent extraction, silical gel chromatography and recrystallization etc.. By elemental analysis and MS (FAB), its formula was determined to be C28H31NO6(MW: 477.2145); based on the UV, IR, 1 HNMR, 13 CNMR, HMQC, HMBC etc. spectral data and physicolchemical properties of SIPI8415, the structure of SIPI8415 was elucidated and was identical with that of spectinabilin, its chemical name is 2methoxy3,5dimethyl6[tetrahydro4[2,4,6trimethyl7(4nitrophenyl)2,4,6heptatrienylidene]2furanyl], 4Hpyran4one.