摘要
研究了以丝纤维为载体，三聚氯氰为交联剂固定化葡萄糖氧化酶膜的方法和条件。实验表明，用二氧六环－二甲苯（质量比１１）为三聚氯氰的溶剂，固定化酶膜效果较好。酶的偶联条件以温度２５℃、ｐＨ＝９、酶液浓度４ｍｇ／ｍｌ为最佳，此时固定化酶膜稳定性好，反复使用３０次酶膜活性保留９０％以上，贮存在离子强度较高的溶液中，酶膜的半衰期为４０～５０周。
A study was carried out on the immobilized methods of glucose oxidase membrane with carrier of silk fabrics and cross linker of tricyanogen chloride. Experimental results showed that the performance of immobilized enzyme membrane was good by using dioxane-dimethyl benzene (1∶1, W/W) as solvent of tricyanogen chloride. The optimum coupling conditions of enzyme are as follows: T=25℃, pH9 and concentration of enzyme 4mg/ml. Reserving in a liquid with higher ionic strength, the half life of enzyme membrane is 40￣50weeks.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1998年第2期140-143,共4页
Chinese Journal of Antibiotics
关键词
固定化酶
丝纤维
葡萄糖氧化酶
酶电极
Immobilized enzyme
Silk fabric
Carrier
Cross linker
Glucose oxidase
