Production of cyclosporin C by Gliomastix luzulae isolated from different areas of the P.R.China

本文调查了１５株粘鞭菌属Ｇｌｉｏｍａｓｔｉｘｌｕｚｕｌａｅ菌种产生环孢菌素Ｃ的能力。分离自中国１１省１５个不同地区的菌种在葡萄糖－麦芽汁－蛋白胨液体培养基中于２４～２６℃发酵１０ｄ。代谢产物经ＨＰＬＣ分析，其中１１株菌株除能产生环孢菌素Ｃ外不产生任何已知的环孢菌素，另４株菌虽然不产生已知的环孢菌素，但全部都产生一未知的环孢菌素。对产生环孢菌素Ｃ的两株具有不同液体培养性状的菌株用Ｌ－丙氨酸、Ｄ－丙氨酸、Ｌ－缬氨酸、ＤＬ－α－氨基丁酸、ＤＬ－原缬氨酸和Ｌ－苏氨酸进行氨基酸前体掺入实验，发现所有氨基酸前体均只能调节菌株合成环孢菌素Ｃ的能力，而不能使其合成相应的其它环孢菌素，其中Ｌ－丙氨酸和Ｄ－丙氨酸具有相近但比其他氨基酸更强的提高菌株合成环孢菌素Ｃ的能力，提示该类菌的合成机制与已知环孢菌素Ａ产生菌的合成机制明显不同。

The capability of fifteen strains of Gliomastix luzulae (Fuckel) Mason et Hughes to produce cyclosporin C was evaluated. Strains from 15 places in 11 Provinces of the P. R. China were cultured on glucose-malt extract-peptone broth for 10 days at 24￣26℃. By HPLC analysis of the extracted samples, 11 of 15 tested strains produced only cyclosporin C without any other known cyclosporins, while all of the other 4 strains produced an unknown cyclosporin. By using two producers of cyclosporin C with different liquid culture characteristics, effect of exogenous amino acids including Lvaline, Lalanine, Dalanine, DLαaminobutyric acid, DLnorvaline and Lthreonine on the formation of cyclosporins was investigated. In all cases, no other cyclosporins except cyclosporin C were produced. Among the tested exogenous amino acids, Dalanine and Lalanine had nearly the same potency on the production of cyclosporin C and both of them were more effective than the other amino acids for the production of cyclosporin C. These results suggest that the biosynthesis mechanism of both strains is quite different from that of Tolypocladium inflatum, a wellknown producer of cyclosporin A.