妇科门诊518例就诊者宫颈拭子HPV检测结果分析

Analysis for cervical swab HPV test results of 518 clients in department of gynecology

目的探讨女性HPV DNA检测在宫颈癌防治方面的意义。方法应用DNA杂交技术对518例妇科门诊就诊者基因分型检测。结果518例样本中,HPV感染有267例,阳性率51.54%。检测高危型HPV(16,18,31,33,35,39,45,51,52,56,58,59,68)218例,占感染病例为81.65%;检出低危型HPV(6,11,42,43,44)42例,占感染病例为15.73%;中国人群常见型HPV(53,66,CP8304)7例,占感染率为2.62%。86例样本中包含了2-5种亚型的感染。结论DNA杂交技术检测HPV基因分型,可1次检测多种亚型,有利于对HPV多重感染的诊断和宫颈癌的防治,可作为宫颈癌筛查的手段。

Objective： To discuss the significance of the detection on women＇s Human Papillomavirus DNA in the prevention and treatment of cervix cancer. Methods： Five hundred and eighteen samples were detected for HPV genotyping by using DNA hybridization technique. Results： In 518 samples , 267 cases were HPV , with the positive rate of 51.54%. 218 cases belonged to high -risk type （HPV - 16, 18, 31, 33, 35, 39, 45, 51,52, 56, 58, 59, 68）, with the ratio of 81.65% in HPV positive cases, 42 cases for low-risk type （HPV-6, 11, 42, 43 , 44）, with the ratio of 15.73% and 7 cases for Chinese common type, with the ratio of 2. 62% . The multi - HPV. - genotype infection was observed in 86 samples. Conclusion： DNA hybridization technique can be used as in this study to simultaneously detect and classify different HPV genotypes , which is useful for the diagnosis of multi - HPV - genotype infection and the prevention and treatment of cervical cancer and may as preliminary means for cervix cancer .