利用CSSL和BIL群体分析稻米垩白率QTL及互作效应

Analysis of QTL and GE Effects on PGWC in Rice (Oryza sativa L.) Using CSSL and BIL Populations

【目的】分析稻米垩白率加性效应、上位性效应及其环境互作效应,探讨稻米垩白率的遗传特点和不同群体检测QTL的效率。【方法】利用由粳稻品种越光和籼稻品种Kasalath杂交衍生的BIL群体和以越光为背景、Kasalath为供体的CSSL群体,对2005年和2006年南京的稻米垩白率QTL及其互作效应进行了分析。【结果】CSSL群体检测到5个垩白率QTL和2对具有上位性效应的QTL;BIL群体检测到3个QTL和4对具有上位性效应的QTL。其中,qPGWC-6a在2个群体中重复出现,1对具有上位性效应的QTL在CSSL群体中2年均被检测到,在BIL群体中,所有QTL与环境存在显著互作(P<0.01)。在第3和4染色体上检测到2个新的垩白率QTL。【结论】上位性效应和加性效应在垩白率遗传中同样重要。垩白率QTL和具有上位性效应的QTL与环境的互作普遍存在,但效应小于相应的加性效应和上位性效应。利用不同群体分析垩白率QTL,有利于全面揭示稻米垩白率的遗传互作网络。

【Objective】 The additive and epistatic and their GE effects on PGWC were analyzed in rice, genetic characters of PGWC in rice and characters of QTL detected in different populations were discussed. 【Method】 A BIL population and a CSSL population derived from the cross of Koshihikari （japonica） and Kasalath （indica） were used to detect QTL and epistatic QTL and GE effects on PGWC in Nanjing in 2005 and 2006. CSSL carried overlapping chromosome segments of Kasalath in a genetic background of Koshihikari. 【Result】 Five QTL and two pairs of epistatic QTL were detected in CSSL population and three QTL and four pairs of epistatic QTL were detected in BIL population. Of them, qPGWC-6a was detected in the two populations. Only one pair of epistatic QTL was detected in two years and in CSSL population. All of QTL had a significant GE effects （P〈0.01） in BIL population. A total of two new QTL for PGWC were found on chromosome 3 and 4 in this experiment. 【Conclusion】 Epistatic effects is important for the heredity of PGWC as well as additive effects, there is ubiquitous for GE effects on PGWC, but which is inferior to the corresponding additive effects and epistatic effects. QTL detected in different populations contributed to completely reveal the genetic network for PGWC in rice.