磁贴穴位敷贴对哮喘患者肺功能及Th1/Th2细胞因子的影响

Effects of Magic Magnetic Plaster Acupoint Application on Lung Function and Th1/Th2 Cytokines in Patients with Asthma

目的:研究曼吉磁贴对哮喘患者肺功能及辅助性T淋巴细胞1(T helper cell 1,Th1)/辅助性T淋巴细胞2(T helper cell 2,Th2)细胞因子的影响。方法:将80例支气管哮喘患者随机分为2组。对照组40例,用丙酸倍氯米松及沙丁胺醇治疗,疗程30 d;治疗组40例,在对照组治疗基础上用曼吉磁贴穴位敷贴治疗,疗程30 d。于1个疗程后判断疗效,观察两组患者给药前后咳嗽症状、昼夜发作次数的变化,并以美国AS-500型肺功能检查仪测定肺功能各项指标,包括一秒钟用力呼气容积(forced expiratory volume in one second,FEV1)、最大呼气流量(peak expiratoryflow,PEF)、最大肺活量(forced vital capacity,FVC);采用酶联免疫吸附测定(enzyme linked im-munosorbent assay,ELISA)检测各组外周血白介素-4(interleukin-4,IL-4)、干扰素-γ(interferon-γ,IFN-γ)水平,采用免疫荧光法检测血清总免疫球蛋白E(total immunoglobulin E,TIgE)水平变化。结果:治疗组支气管哮喘的总有效率为95.00%,其疗效优于对照组(P<0.05)。与治疗前比较,治疗组治疗后症状和肺功能改善明显优于对照组(P<0.05)。两组治疗后IL-4、TIgE水平均显著下降,IFN-γ水平均显著升高。结论:改善患者的肺功能及调节Th1/Th2细胞因子的水平是磁贴穴位敷贴治疗哮喘的可能机制。

Objective： To study the effects of magic magnetic plaster acupoint application on lung func tion and T helper cell 1 （Th1）/T helper cell 2（Th2） cytokines in patients with asthma. Methods： Eighty patients with bronchial asthma were randomly divided into 2 groups. Forty patients in the control group were treated by salbutamol and beelomethasone dipropionate, a 30-day course of treatment; forty ones in the treatment group were treated by aeupoint application of magic magnetic plaster and oral administration of salbutamol and beclomethasone dipropionate, a 30 day course of treatment also. Effects were assessed after one treatment course. The coughing symptoms, the attack number during day and night were observed before and after treatment. The forced expiratory volume in one second （FEV1）, peak expiratory flow （PEF） and forced vital capacity （FVC） were detected by American AS 500 pulmonary function device. The serum levels of interleukin-4 （IL-4）, interferon γ （IFN-γ） were assayed by enzyme labeled immunosorbent assay （ELISA）, and the level of serum total immunoglobulin-E （TIgE） was assayed by immunofluorescence. Results： Compared with those before treatment, the symptoms and lung function after treatment were significantly improved. After treatment, the levels of IL-4 and TIgE in both groups were significantly decreased, and the level of IFN-γ was significantly increased. The total effective rate of the treatment group was 95.00%, being significantly higher than that of control group （P〈0.05）. Conclu sion： The mechanism of magic magnetic plaster acupoint application in treating asthma might be related with improving lung function and regulating the levels of Th1/Th2 cytokines.