猪血小板中蛋白激酶CK2的天然底物

Natural substrates of protein kinase CK2 in pig platelets

目的：寻找猪血小板中蛋白激酶ＣＫ２可能的天然底物。方法：以［γ－３２Ｐ］ＧＴＰ为磷酸供体，用精胺激活蛋白激酶ＣＫ２和用肝素抑制蛋白激酶ＣＫ２活性，然后用放射自显影的方法检测底物蛋白磷酸化。结果：精胺可增加１７．４ｋＤ蛋白磷酸化；肝素能完全抑制４３ｋＤ和６６ｋＤ底物蛋白磷酸化，肝素还增加３９．４ｋＤ底物磷酸化。结论：猪血小板中１７．４ｋＤ。

Objective:To search for natural substrates of protein kinase CK2 in pig platelets.Methods:[γ 32 P]GTP was used as phosphate donor.Activity of protein kinase CK2 was stimulated by spermine and was inhibited by heparin. Protein phosphorylation was detected by autoradiography.Results:Spermine induced 17.4kD polypeptide phosphorylation;heparin completely inhibited 43kD and 66kD polypeptide phosphorylation and also induced 39.4kD polypeptide phosphorylation.Conclusion:17.4kD、43kD and 66kD proteins may be natural substrates of protein kinase CK2 in pig platelets.