几种试剂对镁MTB法自动分析的影响

INFLUENCE OF REAGENTS ON MTB AUTOMATED ANALYSIS OF MAGNESIUM

目的:研究在全自动分析仪上Trig、ALP、CO2等试剂对血清镁测定的影响。方法:分别对混合血清单独测Mg2+和Trig、ALP、CO2等后测Mg2+,比较差异。结果:发现Trig、ALP、CO2试剂均对Mg2+结果产生很大影响,ALP、CO2等试剂使Mg2+测定结果偏高,而Trig使Mg2+测定结果偏低。结论:ALP、CO2等试剂均含有较高浓度的Mg2+致使所测值偏高,而后者Trig致使所测值偏低是由于此试剂成分中含有H2O2显色的物质4-氯酚,其结构中含有OH-和CL-,均属硬碱。而Mg2+属硬酸,根据软硬酸碱理论中所谓"软亲软,硬亲硬"规则4-氯酚与Mg2+结合形成稳定的化合物,从而影响了Mg2+测定,使结果降低。

Objective： To investigate the influence of Trig, ALP and CO2 reagents on MTT automated analysis of magnesium in serum.Methods： To measure magnesium content in mixed serum and compare the results with those obtained after Trig test, ALP test and CO2 test.Results： Trig, ALP and CO2 reagents have great interforenee on the determination of Magnesium content. The application of ALP and CO2 will increase Mg^2＋ content while the use of Trig will reduce the content.Conclusion： Magnesium content goes higher after ALP test and CO2 test because both of the reagents contain much Mg^2＋ in themselves while Mg^2＋ content drops after Trig test for Trig reagent contains 4-CPC, an alkaline substance bringing coloration of H2O2, which can form a stable compound with Mg^2＋, a kind of acid, thus affecting the determination of Magnesium.