腺病毒介导的野生型p53基因转移致血管平滑肌细胞凋亡

Apoptosis of VSMC mediated by wildtype p53 adenovirus gene transference

曾报告腺病毒介导的野生型ｐ５３基因转移能明显抑制体外培养的血管平滑肌细胞（ＶＳＭＣ）的增殖，为探讨ｐ５３基因抑制ＶＳＭＣ增殖的机制，应用电镜、流式细胞计数仪、原位末端标记等方法，观察ｐ５３基因转移对ＶＳＭＣ凋亡的影响。结果证明ｐ５３基因转移可有效地导致ＶＳＭＣ发生凋亡。

It was reported that multiplication of VSMC in vitro could be markedly inhibited by wildtype p53 adenovirus gene.In order to probe the mechanism induced by the inhibition of p53 gene transference.The apoptosis of VSMC was observed by electromicroscope,flowtype cell counter,end labeling in situ and so on.The results showed that p53 gene transference could effectively lead to the apoptosis of VSMC.The study further provided the reliable experimental bases for the gene therapy of cardialvascular disease.