糖基化终产物对人肾小球系膜细胞β1整合素及黏着斑激酶表达的影响

Effects of advanced glycation end products on the expressions of β1-integrin and focal adhesion kinase(FAK)

目的观察糖基化终产物(AGEs)对人肾小球系膜细胞(HRMC)膜上β1整合素及其胞内靶点黏着斑激酶(FAK)表达的影响。方法用不同浓度的AGE-BSA干预培养的HRMC;RT-PCR法测定细胞β1整合素和FAK的mRNA水平;western Blot法检测β1整合素和FAK的蛋白表达。结果在50至400mg/L浓度范围内,AGE-BSA组β1整合素、FAK mRNA及蛋白表达均较对照组增加(P<0.05)。结论一定范围内,AGEs呈浓度依赖性上调β1整合素及其下游信号分子FAK mRNA及蛋白表达,这可能是AGEs致糖尿病肾病的一个重要机制。

Objective To investigate the effects of advanced glycation end products （AGEs） on the expressions of β1-integrin and FAK in cultured human renal mesangiai cells （HRMC）. Methods HRMC were incubated with AGEs-modified bovine serum albumin （AGE-BSA）, the mRNA expressions of β1- integrin and FAK in HRMC were analyzed by use of RT-PCR,and the protein expressions of β1-integrin and FAK in HRMC were determined by Western blot. Results The levels of β1-integrin and FAK mRNA and protein in HRMC incubated with AGE-BSA were significantly higher than those in normal control （P〈0. 05）. Conclusions AGEs up-regulate the expression of β1-integrin and FAK in HRMC, which might have potential implications of the pathogenic mechanism of diabetic nephropathy.