摘要
目的:建立反相高效液相色谱法同时快速测定鹿茸药材中尿嘧啶、次黄嘌呤、尿苷3种生物碱基成分含量。方法:RP-HPLC外标法。采用Agilent TC C18色谱柱(250mm×4.6mm,5μm),流动相为含0.07%醋酸的3%甲醇溶液,流速1.0mL·min-1,检测波长254nm,柱温25℃。结果:在上述色谱条件下,尿嘧啶、次黄嘌呤和尿苷能够得到很好的分离;浓度均在1.7~33.3mg·L-1的范围内与其峰面积线性关系良好,相关系数分别为0.9995,0.9999,0.9996;最低检测限浓度(S/N=3)分别为9.375,18.75,75μg·L-1;平均加样回收率(n=3)在98.4%~103.4%范围内。结论:本方法分离度好,简便快速,重复性好,可用于鹿茸药材中尿嘧啶、次黄嘌呤、尿苷3种生物碱基成分的同时快速测定,具有很高的实用价值。
Objective:To establish an HPLC method for simultaneous and rapid determination of three main biological base components including uracil,hypoxanthine and uridine in antler velvet.Methods:An external standard method with Agilent TC C18 column(250 mm×4.6 mm,5 μm) was adopted with the mobile phase was consisted of 3% methanol-water solution containing 0.07% acetic acid at the flow rate of 1.0 mL·min-1 The detection wavelength was 254 nm and the column was 25 ℃.Results:Uracil,hypoxanthine and uridine were well separated by this method.Linearities of uracil,hypoxanthine and uridine were good(r=0.9995,0.9999,0.9996)in the range of 1.7-33.3 mg·L^-1.The detection limits(S/N=3) were 9.375,18.75 and 75 μg·L^-1 for uracil,hypoxanthine and uridine,respectively.The average recoveries of the three biological base components were found in the range of 98.4%^-103.4%.Conclusion:The method is not only simple,rapid and presents good resolution and reproducibility,but also shows a high utility value,which has been successfully applied to the simultaneous determination of uracil,hypoxanthine and uridine in antler velvet.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第4期575-578,共4页
Chinese Journal of Pharmaceutical Analysis
基金
天津市应用基础研究计划项目(06YFJMJC10500)