胰岛素对高糖状态下血管内皮细胞凋亡、eNOS、bcl-xL、bax表达的影响

Effect of insulin on apoptosis induced by hyperglycemia and on the expressions of eNOS,bcl-xL,bax in vein endothelial cells

高浓度葡萄糖使人脐静脉内皮细胞(HUVEC)凋亡增加,bel-xL和内皮型一氧化氮合酶(eNOS)表达减少;加入生理浓度或高浓度Ins后,HUVEC凋亡显著减少,bcl-xL、bax和eNOS表达增加;HUVEC凋亡与eNOS、bcl-xL、bax表达均呈负相关(r=-0.85,r=-0.81)。表明胰岛素可以抑制高糖引起的HUVEC凋亡,该作用可能与bel-xL、bax和eNOS表达增加有关。

High glucose could increase ECs apoptosis and reduce the expressions of bcl-xL and eNOS. But if physiological or high concentration of insulin was added, the apoptosis of ECs decreased markedly and the expressions of bcl-xL and eNOS increased. The correlation coefficient was - 0. 85 between apoptosis and the expression of eNOS, and -0.81 between apoptosis and the expression of bcl-xL. It showed that insulin can inhibit HUVEC apoptosis induced by hyperglycemia,which may be related with the increased expression of bel-xL and eNOS.