肝脏含铁提取物的制备及对Caco-2细胞吸收铁的促进作用

Preparation of iron content in porcine liver extracts and enhancement of iron uptake in Caco-2 cells

以新鲜猪肝脏为原料,采用中性蛋白酶和风味酶两步水解工艺制备肝脏含铁提取物,经喷雾干燥的提取物粉末的铁含量约为475.3μg/g,且溶解性良好。采用Caco-2细胞作为铁吸收的评价模型,与无机铁FeCl3溶液比较,该含铁提取物粉末具有显著促进铁吸收的作用。这为研究生物利用度高、易于吸收的有机铁提供了一种新来源。

Iron-containing extracts were prepared with the new hydrolytic process from the fresh porcine liver.The protein of the porcine liver extracts was hydrolyzed by two proteinases, neutral proteinase and flavorzyme, and then the extracts were centrifuged ,filtered and spray dried to obtain the iron-containing extract powder.The iron content of the final product was 475.3μg/g with good solubility in water.The iron-containing porcine liver extracts were subsequently tested for effects on iron uptake with Caco-2 cell monolayer.The result showed that it had a significant enhancing effect on iron uptake in Caco-2 cells compared to ferric form-FeCI3 solution,which indicated iron-containing porcine liver extracts,it is a new way to provide a soluble organic iron with high bioavailability.