从大豆糖蜜中分离大豆低聚糖的研究

Study on soybean oligosaccharide separated from soy molasses

以大豆糖蜜为原料,通过单因素考察及正交实验优化了醇沉法提取大豆低聚糖的实验条件,再利用超滤技术除去大豆低聚糖粗提物中大豆乳清蛋白,得到了纯度较高的大豆低聚糖。结果表明:液料比为30:1,提取温度为50℃,乙醇浓度为95%,乙醇浸提时间为2h,低聚糖粗提物得率为55.0%,得到低聚糖产品的纯度为66.3%;超滤纯化低聚糖粗提物后得到低聚糖的纯度为72.2%。

To extract soybean oligosaccharides from soy molasses,the best culture medium of extracted soybean oligosaccharides was confirmed by investigated the single factor and orthogonal experiments.To obtain the best purity of soybean oligosaccharides, ultrafiltration was used to eliminate soybean whey of soybean oligosaccharides.The results showed, the ratio of samples to solvent was 30 ： 1 （ mL/g）, the temperature of extraction was 50℃,the concentration of ethanol was 95% ,the time of ethanol extraction was 2h.The extraction rate of this method was 55.0%, the yield of the production was 66.3%, the purity of soybean oligosaccharides by ultrafiltration was 72.2%.