摘要
以3只瘘管山羊作为瘤胃液供体,用体外法研究添加混合皂苷对瘤胃产气和微生物组成的影响。试验一中茶皂素(茶)和丝兰皂苷(丝)混合添加,添加量分别为底物的0(Ⅰ对照组)、茶0.5%+丝0.01%(Ⅱ组)、茶0.4%+丝0.02%(Ⅲ组)、茶0.3%+丝0.03%(Ⅳ组)、茶0.2%+丝0.04%(Ⅴ组),结果表明,第Ⅳ组添加效果最佳,培养24h时原虫数量极显著低于对照组(P<0.01),并且双毛虫比例有上升趋势。试验二按精粗比不同(20∶80、40∶60、50∶50、60∶40)设计为A、B、C、D,并设添加皂苷组(茶0.3%+丝0.03%)a、b、c、d4组,结果表明,精粗比为40∶60时效果较好,添加皂苷组的产气量极显著高于对照组(P<0.01),且细菌蛋白产量呈上升趋势。
Three goats fisted with cannulas were used to investigate the effects of adding mixtures of saponins on rumen microbes and gas production in vitro.In trial 1 mixtures of tea saponins （T） and yucca saponins （Y） were added into five groups at different levels against subetrates：0 （ Ⅰ ,the control group）,0.5 % T ＋0.01% Y （group Ⅱ ）,0.4 % T＋ 0.02 % Y （group Ⅲ） ,0.3 % T＋03 % Y （group Ⅳ ） ,0.2 % T＋0.04 % Y （group Ⅴ ）.The results indicated that：the test group IV showed optimal effect,protozoa number of 24 h was significantly smaller than that of the control group （P 〈 0.01） ,and diplodinium had an ascending trend.In trial 2 the different dietary ratio concentrate to crude was designed as follows：the control group CA 20：80,B 40：60,C 50：50,D 60：40）,and the test group （a,b,c,d）added tea saponins 0.3 % and yucca saponins 0.03 % respectively.The results showed that the group whose concentrate to crude ratio was 40：60 had better effect,gas production of the test groups added saponins were significantly higher than that of the control group （P 〈 0.01 ）,and the bacteria protein had an increasing trend.
出处
《中国饲料》
北大核心
2009年第8期13-15,共3页
China Feed
关键词
茶皂素
丝兰皂苷
产气量
瘤胃微生物
tea saponin
yucca saponin
gas production
rumen microbes
