病毒性心肌炎NK和LAK细胞活性与心肌损伤的关系

A relationship between NK and LAK cell activities and myocardial injury in viral myocarditis

目的：系统地观察病毒性心肌炎，尤其是柯萨奇Ｂ组病毒（ＣＶＢ）心肌炎患儿自然杀伤细胞（ＮＫ）和淋巴因子激活的杀伤细胞（ＬＡＫ）活性，并分析两者的内在关系，以及与心肌损伤的关系。方法：５６例病毒性心肌炎患儿，年龄３～１２岁，男２６例，女３０例；另设对照组３０例，年龄３～１２岁，男１６例，女１４例。采集静脉血，应用３Ｈ－ＴｄＲ标记法测定ＮＫ和ＬＡＫ细胞活性。结果：（１）心肌炎时ＮＫ和ＬＡＫ细胞活性均明显下降；（２）心肌炎时ＮＫ和ＬＡＫ细胞活性间存在明显的正相关（ｒ＝０．７２，Ｐ＜０．０１）；（３）ＣＶＢ心肌炎患儿的ＮＫ和ＬＡＫ细胞活性下降更显著，且心脏增大和心电图明显异常者发生率亦高于非ＣＶＢ心肌炎者。结论：病毒性心肌炎，尤其是ＣＶＢ心肌炎对ＮＫ及ＬＡＫ细胞活性有明显影响。在病毒性心肌炎诊疗中应同时检测ＮＫ和ＬＡＫ细胞活性。

Objective: In a systemic study of NK and LAK cell activities in viral myocarditis, especially in Coxsackievirus B (CVB) myocarditis, we analysed the relationship between NK and LAK cell activities and between NK and LAK cell activities and myocardial injury. Methods: 56 cases were studied, aged 3～12 years (26 males, 30 females), and 30 of controls(16 males, 14 females), aged 3～12 years. We tested blood NK and LAK cell activities using 3HTdR. Results: (1)Both NK and LAK cell activities decreased in viral myocarditis; (2) There was a good relation between NK and LAK cell activity(r=0.72,P<0.01);(3) NK and LAK cell activities decreased more markedly, and there were high incidences of cardiac enlargement and abnormal electrocardiogram in CVB myocarditis. Conclusion: Viral myocarditis, especially in CVB myocarditis has a significant influence on NK and LAK cell activities. NK and LAK cell activities should be detected simultaneously in the diagnosis and treatment.