甲状腺肿瘤中H-ras基因12密码子和p53基因248密码子突变

Detection of H-ras and p53 gene mutations in human thyroid carcinomas

目的探讨甲状腺癌是否存在H-ras基因12密码子和p53基因248密码子突变。方法用聚合酶链反应—限制性片段长度多态性分析(PCR-RFLP)法分析80例甲状腺肿瘤标本中H-ras基因12密码子和p53基因第248位密码子的突变,包括甲状腺癌54例,甲状腺滤泡性腺瘤10例、结节性甲状腺肿10例和桥本氏甲状腺炎6例。结果 H-ras基因12密码子突变在甲状腺癌为33.3%(18/54),在良性甲状腺病变中为15.3%(4/26),p53基因248密码子突变在甲状腺癌为27.7%(15/54),甲状腺良性病变未检出,两组有显著性差异(P<0.05),24%(13/54)的病例同时存在两种突变。结论甲状腺癌中存在H-ras基因12密码子和p53基因248密码子突变。

Objective To investigate the mutations of H-ras gene codon 12 and p52 gene 248 codon. Methods The samples of 80 cases of thyroid tumors were examined to detect the mutations of H-ras gene codon 12 and p53 gene 248 eodon by using PCR-RFLP. Of the 80 cases, 54 were diagnosed having thyroid carcinoma, including 31 cases of papillary carcinomas, 13 follicular carcinomas, 7 medullary carcinomas and 3 undifferentiated carcinomas. Results Mutations of H-ras gene codon 12 were found in 33.3% (18/54) of thyroid carcinomas and in 15.3% (4/26) of benign diseases, respectively. Point mutations of p53 gene eodon 248 were found in 27.7% (15/54) of thyroid carcinomas and no mutation shown in control tissues. Mutations of H-ras codon 12 and p53 codon 248 coexisted in 24% (13/54) of the patients. Conclusion Point mutations of H-ras and p53 gene existed in thyroid carcinoma, which may cooperatively play an important role in the carcinogenic process of thyroid carcinoma.