神经母细胞瘤细胞周期素D_1的表达检测

Detection of expression of CyclinD_1 gene in neuroblastoma

目的探讨细胞周期素D_1(cyclin D_1)表达与神经母细胞瘤(NB)发生发展的关系及意义。方法应用原位杂交技术对20例NB标本检测。结果 17例可检测到cyclin D_1表达,表达指数(CEI)4.3%～55.3%。年龄<1岁和神经节神经母细胞瘤标本中CEI低于年龄>1岁及NB,但无显著性芳异。Ⅲ～Ⅳ期、UF型及肿瘤广泛转移标本中CEI显著高于Ⅰ～Ⅱ及ⅣS期、F型和肿瘤切除者。CEI与MKI呈正相关,与术后生存时间呈负相关。结论 cyclin D_1能使NB细胞增殖周期加快,与其发生发展关系密切。CEI高,预后差;检测cyclin D_1表达,可为判断NB恶性程度提供实验依据。

Objective To study the relationship between the cyclinD_1 expression and the development of neu-roblastoma (NB) and its significance. Methods 20 cases were detected by in hybridization technique. Re suits Expression of cyclinD_1 was detected in 17 cases. CyclinD_1 expressed index (CEI) were 4.3%—55.3%. CEI was lower in the cases under one year old and gang lioneuroblastoma (GNB) than in the cases over one year and NB with the difference being not significant. It was significantly higher in the cases with UF type,stage Ⅲ or Ⅳ and metastatic tumor than those with F type,stage Ⅰ or Ⅱ or ⅣS,total resection of tumor or primary tumor. There was positive correlation between CEI and mitotic karyorrheksi index (MKI). There was negative correlation between CEI and survival time. Conclusion The development of NB may be associated with cyclinD_1 expression. The prognosis was poor in the cases with a higher CEI. Detection of cyclinD_1 expression may be used as a measure for predicting biological behavior and prognosis of NB.