苯对小鼠骨髓细胞p53表达的影响

p53 expression in benzene inhalation of mice

目的：探讨苯对小鼠骨髓细胞ＤＮＡ基因的毒性作用。方法：采用静式吸入染毒３０ｄ，用免疫组化方法测定小鼠骨髓细胞ｐ５３阳性率，并作ｕ检验。结果：染毒３０ｄ后无一小鼠死亡，白细胞计数也在正常范围内，而ｐ５３表达则是最高浓度组高于对照组和其它染毒组（Ｐ＜０．０１或Ｐ＜０．０５）。结论：苯吸入对小鼠的骨髓细胞ＤＮＡ有一定的影响，可能是引起白血病的原因之一。

Objective:To study the toxicity of benzene inhalation on DNA of bone marrow cells in mice.Methods:After static inhalation of benzene for 30 days,bone marrow cells were tested for p53 by using immunohistochemical technic,and u test is calculated for comparison with the control group.Results:All experimental mice all survived,their white cells counts were within normal range.p53 expression of the highest concentration inhaling group(3.96 mg/L) was higher than that of the control and the other experimental groups(P<0.01 and P<0.05).Conclusions:Inhalation of benzene effects DNA of bone marrow cells.This may be one of causes of leukemia.