猪瘟与猪圆环病毒2型混合感染检测方法的建立与初步应用

The Establishment and Preliminary Application of Detection Method of Mixed Infection both Classical Swine Fever and Porcine Circovirus Type 2

根据GenBank上发表的猪瘟（classical swine fever virus，CSFV）和猪圆环病毒2型（porcine circovirustype2，PCV2）的核苷酸序列，应用NCBI／Primer Blast设计合成分别针对CSFV和PCV2的1对特异性引物，建立了分别用于检测CSFV和PCV2的RT—PCR和PCR方法，并对2008年4月～8月采自昆明8个可疑发病猪场的19份病料进行了CSFV和PCV2检测。结果显示，来自3个猪场的4份检测样品为CSFV阳性，猪场的CSFV阳性率为21．05％；来自2个猪场的2份检测样品为PCV2阳性，猪场的PCV2阳性率为10．53％。CSFV和PCV2混合感染率为10．35％。表明所建立的CSFV和PCV2混合感染检测方法具有特异性，可用于CSFV和PCV2混合感染的辅助诊断和流行病学调查，同时也为CSFV和PCV2混合感染的防制提供科学的依据。

According to the published nucleotide sequence of CSFV and PCV2 in GenBank, one pair of specific primers of nucleotide sequence of CSFV and PCV2 were designed respectively by NCBI/Prime-BLAST and synthesized. The PCR and RT PCR method to PCV2 and CSFV was established, and 19 clinical samples collected from eight pig farms in Kunming from April to August in 2008 were detected to CSFV and PCV2. The results showed that, four samples from three farms were positive CSFV, the infection rate of positive CSFV was 21.05 percent; two samples from two farms were positive PCV2, the infection rate of positive PCV2 was 10.53 percent. CSFV and PCV2 mixed infection rate was 10.53 percent. The established detection method of mixed infection both CSFV and PCV2 was specific, and can be used for detecting CSFV and PCV2 mixed infection and epidemiological investigation, and can also provided scientific basis for prevention and control of CSFV and PCV2.