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微流控技术在生殖领域中的应用 被引量：1

Research Progress on the Application of Microfluidics in Reproduction
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摘要 微流控技术(microfluidics)是微全分析系统(micro total analysis system,μTAS)和芯片实验室(lab-on-a-chip)的重要支撑技术,其微量化和微尺度的优势得到人们越来越多的关注。辅助生殖技术(assisted reproductive technology,ART)是生殖医学的重要组成部分,已被广泛应用于生产实践中,但由于其低效性,人们在不断寻求新的技术手段。笔者综述了近年来微流控技术在生殖领域中的应用,包括精子筛选、体外受精和配子/胚胎培养等方面的内容,为辅助生殖技术提供了新的平台。 Microfluidics is the important supporting technology of μTAS and lab-on-a-chip, whose microanalysis and microscale has obtained much more attention. Assisted reproductive technology （ART）, as an important part of reproductive medicine, has already been put into practice widely. Owing to the low effectiveness of ART, new technical means are required to put in use. This paper presents a review of the application of microfluidics in reproduction during recent years, including sperm isolation, in vitro fertilization （IVF） and gamete/embryo culture. Mierofluidies provides new platform and prospect for ART.
作者 孙子龙 寒超 马睿 张建海 牛瑞燕 程京 王俊东
机构地区 山西农业大学山西省生态畜牧与环境兽医学重点实验室 山西农业大学动物科技学院动物医学系 清华大学医学院医学系统生物学研究中心 生物芯片北京国家工程研究中心
出处 《中国畜牧兽医》 CAS 北大核心 2009年第4期92-95,共4页 China Animal Husbandry & Veterinary Medicine
关键词 微流控技术 辅助生殖技术 精子筛选 体外受精 配子/胚胎培养 microfluidics ART sperm isolation IVF gamete/embryo culture
分类号 S818.9 [农业科学—畜牧学]
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参考文献25

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同被引文献8

  • 1Funahashi H. Polyspermic penetration in porcine IVM - IVF systems. Reprod Fertil Dev, 2003, 15 : 167 - 177.
  • 2Hikaru SANO, Koji MATSUURA, Keiji NARUSE, et al. Application of a microfluidic sperm sorter to the in - vitro fertilization of porcine oocytes reduced the incidence of polyspermic penetration. Theriogenology, 2010, 74 (5): 863-870.
  • 3Koji Matsuura, Mami Takenami, Yuka Kuroda, et al. Screening of sperm velocity by fluid mechanical charac- teristics of a cyclo - olefin polymer microfluidic sperm - sorting device. Reproductive BioMedicine Online, 2012, 24, 109-115.
  • 4Hyakutake T, Hashimoto Y, Yanase S, et al. Appli- cation of a numerical simulation to improve the separation efficiency of a sperm sorter. Biomed. Microdeviees. 2009, 11, 25-33.
  • 5Rath D. Experiments to improve in vitro fertilization techniques for in vivo - matured porcine oocytes. Ther- iogenology 1992 ; 37 : 885 - 896.
  • 6Alminana C, Gil MA, Cuello C, et al. Adjustments in IVF system for individual boars: value of additives and time of sperm - oocyte co - incubation. Theriogenology 2005 ; 64 : 1783 - 1796.
  • 7Li YH, Ma W, Li M, et al. Reduced polyspermic penetration in porcine oocytes inseminated in a new in vitro fertilization (IVF) system: Straw IVF. Biol Re-prod 2003 ; 69 : 1580 - 1585.
  • 8Toru Hyakutake, Yuki Hashimoto, Shinichiro Yanase, et al. Application of a Numerical Simulation to Improve the Separation Efficiency of a Sperm Sorter Biomedical Microdevices, 11 (2009), 25 - 33.

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中国畜牧兽医
2009年 第4期

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