4个绵羊品种双肌臀基因基因型的检测

Detection of Four Sheep Breeds Callipyge Genotype

绵羊的双肌臀(Callipyge,CLPG)基因表型为后臀肌肉增大近30%,是由位于绵羊18号染色体GTL2基因上游32.8 kb处存在1个A→G的单核苷酸多态性(SNP)标记(命名为SNPCLPG)产生的,该SNP标记的存在与CLPG表型完全吻合。通过对SNPCLPG的分子检测,期望发现CLPG基因型种羊,为今后选育肉用性状更优异的新品系奠定基础。为此,选择设计合成了1对引物,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法,分别以引进的纯种陶塞特羊、特克塞尔羊、夏洛莱羊和美利奴羊作为研究样本,进行了SNPCLPG位点的PCR检测。获得了预期的493 bp扩增片段,并检测到CLPG基因型特征性的SNPCLPG突变(A→G)。

The buttocks muscle can be increased about 30% by Callipyge （CLPG）, resulting from an apparent A→G mutation in 32.8 kb upstream of GTL2 in the ovine chromosome 18. The mutation polymorphism is referred to as SNPcleon, which was later shown to be the only difference between the callipyge and wild-type chromosome. The objective of the research is to select carriers of the callipyge gene by the molecular biological techniques, which is the basis of selection of the excellent meat traits. The SNPs were detected by PCR-restriction fragment length polymorphisms （PCR-RFLP） with one pairs of primers in genome of Dorset sheep, Texel sheep, Charolais sheep and Merino sheep. A→G substitution was found and one special products （493 bp） were amplified.