桦褐孔菌诱变菌株的拮抗试验及酯酶同工酶检测

Tests of Mutant Strains of Inonqqus oblique by Antagonistic Actions and Esterase Isoenzymes

通过^(60)Co-γ辐射诱变桦褐孔菌菌株,对诱变菌株进行拮抗试验,检测其与原始菌株有无拮抗现象;并采用聚丙烯酰胺凝胶电泳技术对诱变菌株进行酯酶同工酶分析,通过netsys软件对各菌株与原始菌株的差异性进行了聚类分析。结果表明:诱变菌株均无拮抗现象;但各诱变菌株的酯酶同工酶酶谱有一定差异。

The mutants of Inonqqus oblique treated by radiation of ^60Co-γ were tested antagonistic actions with original strain. Then the esterase isoenzymes from the mutant strains were studied by using electrophoresis of polyacrylamide gel and cluster analysis were carried out on the diversities between mutants and original strains by netsys. The results showed that all mutant strains had no antagonistic actions with original strain 410 ;the esterase isoenzymes were different between mutants and original strain.