脑脊液中脑膜癌细胞CEA表达的研究

Observation of CEA in meningeal carcinomatosis cells of cerebrospinal fluid by laser scanning confocal microscopy

目的联合应用免疫荧光细胞化学技术和激光扫描共聚焦显微镜技术(LSCM)检测脑脊液中脑膜癌细胞CEA的表达部位并定量测定其荧光含量,探讨该技术在脑膜癌病诊断中的价值。方法应用免疫荧光细胞化学法以CEA标记脑膜癌细胞及对照组脑脊液细胞,采用LSCM技术获得脑膜癌细胞扫描图像并定量测定CEA荧光强度值。结果脑脊液中脑膜癌细胞的CEA表达为阳性,且部位在细胞浆中;CEA平均荧光值(36.80±17.17),与对照组(4.87±1.86)相比差异有统计学意义(P<0.01)。结论联合应用免疫荧光细胞化学法和LSCM检测脑脊液细胞中CEA的表达可为脑膜癌病的诊断提供可靠依据。

Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy （ LSCM ）, explore the diagnostic value of the examination in the patients with meningeal carcinomatosis. Methods All cerebrospinal fluid cells （ control group and meningeal carcinomatosis ） were stained with CEA by immunofluorescenee, scanogram and fluorescent intensity stereophotography were obtained and finally fluorescent values of CEA were individually measured by LSCM. Results Compared with meningeal carcinomatosis cells （44.33 ± 29.58） and control group cells （2.37 ± 2.16）, fluorescent value difference CEA was statistically significant （P 〈 0.01 ）. Conclusion The sensitivity of CEA by immunofluorescence examination and LSCM is useful for the diagnosis, differential diagnosis of meningeal carcinomatosis.