转化生长因子β1(TGF-β1)在胃癌组织中的表达

Study of expression of Transforming growth factor beta 1 in gastric carcinoma tissue

目的检测转化生长因子β1(transforming growth factor beta 1,TGF-β1)在胃癌组织及其相应正常胃黏膜组织中的表达,并分析其与胃癌患者临床病理学特征之间的关系。方法选取39例经外科手术切除及组织病理学证实的胃癌患者的癌组织标本及相应的正常胃黏膜组织,应用RT-PCR技术和冰冻切片免疫组化染色方法,分别在mRNA和蛋白水平检测TGF-β1的表达。结果30例胃癌组织有TGF-β1的阳性表达。在临床Ⅰ期和Ⅱ、Ⅲ、Ⅳ期两组以及浸润深度为T1和T2、T3、T4两组胃癌患者的癌组织中TGF-β1阳性表达差异有统计学意义(P<0.05)。胃印戒细胞癌胞浆频繁出现TGF-β1强阳性染色。结论TGF-β1在胃癌发生、发展中发挥着重要作用,TGF-β1可成为早期胃癌诊断、治疗和预后的分子标志。

Objective To detect the expression and disposition of TGF beta 1 in the gastric carcinoma tissue and the corresponding normal mucosal tissue, and to analyze the relations between the expression, disposition and the clinieopathologic characteristics. Methods 39 cases of gastric cancer and normal tissues confirmed by histopathology were selected to detect and compare in the level of mRNA and protein by means of RT - PCR and frozen section immunohistochemistry staining technology. Results 30 cases （76.9%） were positive for TGF beta 1 mRNA expression and 23 cases were positive for TGF beta 1 staining. There are significance between stage Ⅰ group and stage Ⅱ、Ⅲ、Ⅳ group as far as TGF beta 1 expression is concerned , the same to T1 depth of invasion and T2 , T3 , T4 depth of invasion （P 〈 0.05 ）. Frequently, strong TGF beta 1 staining Occurred in gastric signet -ring cell carcinoma. Conclusion The TGF beta 1 plays an important role in the pathogenesis of gastric carcinoma. It appears to be a molecule marker in the early diagnose,management and prognostic about gastric carnoma.