中药预知子乙醇提取物抗抑郁作用的实验研究

Studies on the anti-depressant effects of Akebia fruit alcohol extract

目的:研究预知子乙醇提取物的抗抑郁作用,并初步探讨其作用机制。方法:采用小鼠悬尾实验、强迫游泳实验,抑制单胺重摄取作用实验等动物模型来考察预知子乙醇提取物抗抑郁作用及其作用机制。结果:预知子乙醇提取物能够显著缩短小鼠悬尾及强迫游泳不动时间;显著增加多巴胺(DA)致小鼠死亡作用和阿朴吗啡致小鼠刻板运动作用;增加5-羟色胺酸(5-HTP)致甩头作用,但对去甲肾上腺素(NE)重摄取抑制作用不明显。结论:预知子乙醇提取物有抗抑郁活性,与其增强5-HT、DA神经系统作用有关。

Objective： To investigate the anti-depressant effects of Akebia fruit alcohol extract and to explore its mechanism. Methods ：Tail suspension model and force-swim model of mice were used to observe the effect of Akebia fruit alcohol extract on relieving depression. The mechanism was examined by inhibiting reuptake of monoamine neurotransmitters. Results：Akebia fruit aleohol extract can reduced the immobility time in both of the tail suspension test and the forced swim test of mice . Akebia fruit alcohol extract significantly increased the rate of death of injecting dopamine and enhanced the apomorphine-induced stereotypy, could increase the head-twitch response evoked by 5-HTP, but it had no direct activation on reuptake of noradrenalin . Conclusion：Akebia fruit alcohol extract has antidepressant effects ,and its mechanism may be related to the reinforcement of 5-HT nerves system and DA nerves system.