高原地区先天性心脏病患者核心家庭血清叶酸、维生素B_(12)、同型半胱氨酸水平及民族相关性研究

RELATIONS BETWEEN SERUM HOMOCYSTEINE And FOLIC ACID LEVELS WITH CONGENITAL HEART DISEASE IN ALTIPLANO

目的了解高原地区先天性心脏病(CHD)患者血清叶酸(FA)、维生素B12(VB12)、同型半胱氨酸总水平(tHCY)与其父母血清中三者水平的相关性,及不同民族间的差异性。方法选取CHD患者及其生物学父母作为病例组,另选取西宁地区无出生缺陷病史及家族史的正常人及其生物学父母作为相应对照。采用化学发光免疫法测定全部对象血清FA、VB12及tHCY水平。结果CHD患者血清中tHCY含量明显低于正常对照者(P>0.05),而FA、VB12含量明显高于正常对照组,且CHD病例组血清中tHCY、FA、VB12水平同其生物学父母血清中此三项指标无相关性(P>0.05)。藏族CHD患者血清中tHCY、VB12水平明显低于汉族和回族患者(P<0.05)。结论高原地区先天性心脏病患者核心家庭血清FA、VB12、tHCY水平同CHD的相关性尚不能定论,CHD患者血清中tHCY、VB12水平可能存在民族差异。

Objective To investigate the relations among serum homocysteine （HCY）, vitamin B12 （VB12）and folic acid （FA） with congenital heart disease （CHD） in CHD nuclear families, and the difference of these index among different nationalities live in altiplano. Methods CHD patients and their biological parents were selected as the case group, and another normal subjects and their parents as control. For all members the serum levels of total HCY （tHCY）, folic acid and vitamin B12 （VB12） were determined by chemiluminescence immunoassay. Results compared with the control group, the levels of tHCY in CHD patients serum were obviously lower（ P 〈0.05）, and the levels of folic acid ,VB12 were obviously higher. In CHD patients, the levels of tHCY, VB12, folic acid was not associated with their biological parents（P 〉0.05）. The levels of tHCY,VB12 in Tibetan CHD patients were obviously lower than that of in Han and Hui CHD patients. Conclusion In this study, the folic acid and tHCY were not significantly related with CHD and it needs further investigations to confirm it. There were obvious nationality distinctions on the levels of tHCY, VB12 in CHD patient＇s serum.