摘要
目的建立三七总皂苷缓释片中人参皂苷Rg1的含量测定方法。方法采用RP-HPLC法,色谱柱为Hyper-sil ODS C18柱(4.6mm×250mm,5μm);流动相:以乙腈-水梯度洗脱;流速:1.0mL/min;紫外检测波长:203nm;柱温:25℃。结果人参皂苷Rg1的线性范围为0.1028-0.5140mg/mL;相关系数为0.9999;加样平均回收率为97.74%(RSD=0.79%)。结论该法操作简便,结果准确,重复性好,可作为该制剂的质量控制方法。
Objective To establish a method for quantitative determination of Ginsenosides Rgl in sustained - release tables of panax notog/nsenos/des. Method RP - HPLC was apphed for the determination. The chromatographic column was Hypersil ODS C l8 （4.6mm × 250 mm, 5 μm）, the mabilephase was acetonitrile - water gradient elution, the flow rate was 1.0ml/min; the detection wavelength was set at 203 nm; temperature was 25℃. Results The linearity ranges of Ginsenosides Rgl was 0. 1028 -0.5140mg/ml, the correlation was 0.9999 ;the average recovery rate of adding sample was 97.74% （ RSD = 0.79% ）. Conclusion The operation is simple, the result is accurate, the reproductability is good. It is for the quality control of sustained - release tables of panax notoginsenosides.
出处
《湖北中医学院学报》
2009年第2期28-30,共3页
Journal of Hubei College of Traditional Chinese Medicine
基金
湖北省教育厅技术研究项目
[D200716005]
