日本矮生沿阶草愈伤组织的诱导及其分化

Study on callus induction and plantlet regeneration of Ophiopogon japonicus

选用不同外植体和不同培养基,对日本矮生沿阶草Ophiopogon japonicus进行了愈伤组织的诱导及分化的研究。结果表明:幼嫩叶片的基部是最适宜诱导愈伤组织的外植体。G3(MS+KT0.2mg/L+2,4-D0.1mg/L)培养基的愈伤组织的诱导率达到89%以上,将诱导的愈伤组织置于J1(MS+BA2.0mg/L+NAA0.2mg/L)培养基中的愈伤组织的分化率最高,达到59%。因此,选用幼嫩的叶片基部置于G3培养基进行诱导,继代一次后置于J1培养基进行分化是最有利于再生的途径。

The differentiation of Ophiopogon japonicus callus from different explants with different medium was conducted. The result showed that the base part of young leaf was best for callus induction. and the induction rate was more than 89% in G3 medium （MS＋KT 0.2 mg/L ＋2,4-D 0. 1 mg/L）, and the differentiation rate could reach 59% in J1 medium （MS ＋BA 2.0 mg/L ＋NAA 0.2 rag/L）.