缬沙坦对人脐动脉平滑肌细胞PAPP-A、IGF-1表达的影响

Influence of valsartan on PAPP-A,IGF-1 gene expression in HUVSMC

目的:探讨缬沙坦对人脐动脉平滑肌细胞PAPP-A、IGF-1表达的影响。方法:分别在10-5mol/L的Ox-LDL的基础上,给予AngII以及AngII、缬沙坦混合培养人脐动脉平滑肌细胞,以普通培养基培养为对照,应用ELISA及RT-PCR的方法分别检测上清液及细胞内PAPP-A、IGF-1的表达量。结果:AngII组PAPP-A与IGF-1的表达量均显著高于对照组、Ox-LDL及缬沙坦组(P<0.01),缬沙坦组IGF-1的表达量及PAPP-ARNA的表达量与Ox-LDL组无明显差异(P>0.05),而缬沙坦组细胞外PAPP-A分泌量则与其它各组均差异明显(P<0.01)。结论:AngII可促进人脐动脉平滑肌细胞PAPP-A和IGF-1的表达,缬沙坦可以抑制AngII的这种作用,缬沙坦对AngII作用下PAPP-A细胞内RNA表达的抑制较细胞外蛋白分泌的抑制明显。

Objective ： To study the effect of Valsartan on the expressions of the PAPP-A, IGF-1 in human umbilical vein smooth muscle cell. Methods： Under the condition of ox-LDL of 10^-5 moL/L, HUVSMC is cultured with Ang Ⅱ , and with the combination of Aug Ⅱ and Valsartau. Compared with normal cultivation without ox-LDL, the expressions of PAPP-A, IGF-1 are assayed using RT-PCR and ELISA. Results： The expressions of PAPP-A and IGF-1 that Ang Ⅱ induces are significantly higher than those in other groups. The Valsartan group makes no significant differences compared with the ox-LDL group in the mRNA expressions of PAPP-A and IGF-1. The ex-cell expression of PAPP-A that Valsartan induces has significant difference compared with ones in other groups. Conclusion： Aug Ⅱ induces the expressions of PAPP-A, IGF-1 while Valsartan can deregulate the role that Ang Ⅱ plays, especially through the ex-cell pathway.