期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

FHL2调控Id2功能活性的研究

Role of FHL2 in regulation of Id2 functional activity
下载PDF
导出
摘要 目的:探讨FHL2对Id2蛋白功能的调控效应。方法:将E47、5xE-Box-Luc、pRL-SV40、Id2以及FHL2表达载体分别共转染MCF-7、293T以及SH-SY5Y细胞,双荧光素酶报告基因方法检测不同转染组细胞中的相对荧光素酶活性。结果:三个细胞系中E47均显著上调了报告基因的表达,Id2抑制了E47的转录激活活性,而FHL2通过与Id2相互作用显著消弱了Id2对E47功能的抑制效应。结论:通过相互作用FHL2抑制了Id2蛋白的功能活性,FHL2是一个新的Id2蛋白功能抑制子。 Objective： To study the role of FHL2 in regulation of Id2 functional activity. Methods： Expression vectors including FAT, 5xE Box Luc, pRL SV40, Id2 and FHL2 were cotransfected with MCF-7, 293T and SH-SY5Y cells, respectively. Relative luciferase values for transfected cells were measured by dual luciferase report assay. Results： FAT significantly upregulated the expression of reporter gene in three cell lines. Id2 inhibited the activity of FAT. FHL2 decreased the inhibitory effect of Id2 by interacting with Id2. Conclusion ： FHL2 is a novel functional repressor of Id2 by suppressing the functional activity of Id2.
作者 伍志强 韩为东 赵亚力 司艺玲 杨洁 田丽媛 孟元光
机构地区 解放军总医院基础所分子生物室 解放军总医院基础所免疫室 解放军总医院妇产科
出处 《军医进修学院学报》 CAS 2009年第2期197-199,共3页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金资助项目(30670809) 解放军总医院苗圃基金资助(07MP69)~~
关键词 FHL2 ID2 蛋白质相互作用域和基序 阻遏蛋白质类 FHL2 Id2 protein interaelion domains and motifs repressor proteins
分类号 R349.64 [医药卫生—基础医学]
  • 相关文献

参考文献9

  • 1Massari ME, Murre C. Helix-loop-helix proteins: Regulators of transeription in eucaryotic organisms [ J ]. Mol Cell Biol, 2000, 20 ( 2 ) :429--440.
  • 2伍志强,孟元光,赵亚力,杨洁,田丽媛,司艺玲,韩为东.HLH缺失型Id2候选相互作用蛋白[J].军事医学科学院院刊,2007,31(4):317-321. 被引量：5
  • 3Fong S, Debs RJ. Desprez PY. Id genes and proteins as promising targets in cancer therapy [J]. Trends Mol Med, 2004,10(9):387-392.
  • 4Ruzinova MB, Benezra R. Id proteins in development, cell cycle and cancer[J].Trends Cell Biol, 2003, 13(8) :410-418.
  • 5Lasorella A, lavarone A. The protein ENH is a cytoplasmic sequestration factor for Id2 in normal and tumor cells from the nervous system[J].Proc Natl Acad Sci USA, 2006, 103(13) :4976-4981.
  • 6Benezra R. Polycystins : inhibiting the inhibitors[ J ]. Nature Cell Biol, 2005, 7(12) :1164-1165.
  • 7lavarone I, Lasorella A. ID proteins as targets in cancer and tools in neurobiology[J]. Trends Mol Med, 2006. 12(12) :588-594.
  • 8Johannessena M, M llera S, Hansena T, et al. The muhifunctional roles of the four-and-a-half-LIM only protein FHL2 [J]. Cell Mol Life Sci, 2006, 63(3) :268-284.
  • 9Kleiber K, Strebhardt K, Martin BT. The biological relevance of FHL2 in tumor cells and its role as a putative cancer target [ J ]. Anticancer Res, 2007, 27 (12):55-62.

二级参考文献22

  • 1Benezra R,Davis RL,Lockshon D,et al.The protein Id:a negative regulator of helix-loop-helix DNA binding proteins[J].Cell,1990,61(1):49-59.
  • 2Yokota Y,Mansouri A,Mori S,et al.Development of peripheral lymphoid organs and natural killer cells depends on the helix-loop-helix inhibitor Id2[J].Nature,1999,397(6721):702-706.
  • 3Mori S,Nishikawa S,Yokota Y.Lactation defect in mice lacking the helix-loop-helix inhibitor Id2[J].EMBO J,2000,19(21):5772-5781.
  • 4Perk J,Iavarone A,Benezra R.Id Family of helix-loop-helix proteins in cancer[J].Nat Rev Cancer,2005,5(8):603-614.
  • 5Lasorella A,Iavarone A.The protein ENH is a cytoplasmic sequestration factor for Id2 in normal and tumor cells from the nervous system[J].Proc Natl Acad Sci USA,2006,103(13):4976-4981.
  • 6Kurooka H,Yokota Y.Nucleo-cytoplasmic shuttling of Id2,a negative regulator of basic helix-loop-helix transcription factors[J].J Biol Chem,2005,280(6):4313-4320.
  • 7Wang S,Sdrulla A,Johnson JE,et al.A role for the helix-loop-helix protein Id2 in the control of oligodendrocyte development[J].Neuron,2001,29(3):603-614.
  • 8Lasorella A,Stegmüller J,Guardavaccaro D,et al.Degradation of Id2 by the anaphase-promoting complex couples cell cycle exit and axonal growth[J].Nature,2006,442(7101):471-474.
  • 9Lasorella A,Noseda M,Beyna M,et al.Id2 Is a retinoblastoma protein target and mediates signalling by Myc oncoproteins[J].Nature,2000,407(6804):592-598.
  • 10Park HR,Jung WW,Kim HS,et al.Upregulation of the oncogenic helix-loop-helix protein Id2 in Ewing sarcoma[J].Tumori,2006,92(3):236-240.

共引文献4

军医进修学院学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部