丙泊酚对毒鼠强诱发大鼠癫痫的抑制作用及机理

Inhibitory effects of propofol on tetramine-induced epilepsy in rats and its mechanism

目的:观察丙泊酚对毒鼠强诱发癫痫的抑制作用及其机理的实验研究。方法:64只大鼠分为对照组,癫痫发作组,脂肪乳组,安定组,丙泊酚25、50、75、100mg/kg剂量组,观察不同剂量的丙泊酚对惊厥的控制、24h的存活率及脑电图变化。以WesternBlot法观察麻醉剂量丙泊酚对癫痫大鼠皮层和海马GABAA受体α1亚单位表达的影响。结果:丙泊酚25～100mg/kg可剂量依赖性的控制癫痫大发作,增加24h的存活率。丙泊酚25mg/kg剂量组,脑电图仍见明显的高幅棘波;50～100mg/kg剂量组棘波明显减少,波幅显著降低。WesternBlot结果:丙泊酚50mg/kg显著增加GABAA受体α1表达。结论:丙泊酚对毒鼠强诱发癫痫持续状态具有确切的治疗效果,其抗惊厥作用可能与其增加GABAA受体α1亚单位表达有关。

Objective： To observe the inhibitory effect of propofol on tetramine -induced epilepsy in rats and its mechanism. Methods： Sixty-four SD rats were divided into blank group, epilepsy group, and intralipid, diazepam and propofol （25, 50, 75, 100mg/kg） groups. EEG was recorded for 5h and survival rate after 24h, was used as a criterion for observing the inhibitory effect of propofol on epilepsy in rats. Western blotting was employed to determine the cortical and hippocampal expression of GABAA receptor α1 subunit in rats with epilepsy. Results： Propofol improved the seizure of epilepsy in a dose dependent-manner. Compared with epilepsy and intralipid groups, the frequency and amplitude were significantly decreased in the other three groups. Propofol at a dose of 50mg/kg could significantly up-regulate the expression of GABAA receptor α1 subunit. Conclusion： Propofol can effectively inhibit tetramine-induced epilepsy by up-regulating the expression of GABAA receptor α1 subunit.