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FHL2与Id蛋白相互作用的表位分析

Epitope analysis of interaction between FHL2 and Id proteins
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摘要 目的:鉴定FHL2与Id家族蛋白成员之间的相互作用及分子表位。方法:GST-pulldown方法检测。结果:FHL2与Id家族的4个成员蛋白均存在直接的相互作用关系,FHL2蛋白中的第二个LIM结构域在FHL2/Id相互作用中是必需的,Id蛋白N端结构区域在介导FHL2/Id相互作用中是必需的。结论:FHL2是一个新识别的Id蛋白广谱相互作用因子,FHL2可能参与Id介导的多种生物学效应以及肿瘤发生与进展。 Objective： To investigate the interaction between FHL2 and Id proteins and its corresponding epitope. Methods： Interaction between FHL2 and Id proteins and the corresponding epitope conferring this association were detected by GST pulldown assays. Results： FHL2 directly interacted with all Id proteins. The second LIM domain containing FHI2 or N ter- minal region in Id proteins was essential for the direct binding of FHL2 to ld proteins. Conclusion： FHL2 is a novel and broad interacting factor of the ld proteins. Through cooperating with Id proteins, FHL2 may be involved in multiple biological effects and tumorigenesis mediated by Id proteins.
作者 韩为东 赵亚力 伍志强 司艺玲 杨洁 田丽媛 孟元光
机构地区 解放军总医院基础所分子生物室 解放军总医院基础所免疫室 解放军总医院妇产科
出处 《军医进修学院学报》 CAS 2009年第2期206-208,共3页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金资助项目(30870507) 解放军总医院苗圃基金(07MP69)~~
关键词 FHL2 ID 基因表达调控 蛋白质相互作用图 FHL2 Id gene expression regulation protein interaction mapping
分类号 R349.64 [医药卫生—基础医学]
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参考文献5

  • 1Massari ME, Murre C. Helix-loop-helix proteins: Regulators of transcription in eucaryotic organisms [ J ]. Mol Cell Biol, 2000, 20(2 ) :429-440.
  • 2伍志强,孟元光,赵亚力,杨洁,田丽媛,司艺玲,韩为东.HLH缺失型Id2候选相互作用蛋白[J].军事医学科学院院刊,2007,31(4):317-321. 被引量：5
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二级参考文献22

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军医进修学院学报
2009年 第2期

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