摘要
目的研究肺腺癌患者外周血中Ephb4启动子区域甲基化状态，发现Ephb4甲基化与肺腺癌之间的关系。方法采用甲基化特异性PCR（MSP）方法检测肺腺癌样本、及对照正常人血样本中Ephb4基因启动子区甲基化表型状态。结果证明Ephb4基因启动子区域在肺腺癌血样本中呈现高甲基化状态23．8％，正常人血样本中为O％。结论Ephb4基因启动子区域呈现高甲基化状态，表明Ephb4基因可能是肺腺癌的发生的早期事件，在肺腺癌的发生发展过程中起重要作用。
Objective To analyze the promoter region methylation of Ephb4 gene in blood from the adenocarcinoma of lung patients for finding their relation.Methods Methylation-specific PCR（MSP） was used for the detection of promoter region hypermethylation in blood DNA from adenocarcinoma of lung and normal blood samples.Results The incidence of the hypermethylation of Ephb4 promoter region in blood from of NSCLC patients was 23.8%, no methylated Ephb4 gene promoter was found in the normal samples（0%）.Conclusion We detect the aberrant methylation of Ephb4 gene in blood from adenocarcinoma of lung patients. In our present study, Ephb4 may be an early event of adenocarcinoma of lung and may contribute to the adenocarcinoma of lung.
出处
《中国血液流变学杂志》
CAS
2009年第1期10-12,共3页
Chinese Journal of Hemorheology
基金
国家自然科学基金资助项目（30672400）
