摘要
利用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术对返回式卫星搭载两个小麦品种SP3代籽粒的高分子麦谷蛋白亚基(HMW-GS)进行了分析,并按照相关评分方法计算了高分子量麦谷蛋白Glu-1位点的品质得分。结果表明,经卫星搭载可产生较高频率的HMW-GS基因变异,陕253和西农1043两个品种SP3代HMW-GS组成的变异频率分别为27.08%和27.45%。陕253和西农1043SP3代的小麦品质得分分别为7分和6分,陕253SP3代变异株为优质小麦。
The compositions of high molecular weight glutenin subunits （HMW-GS）of the third generation （SP3） of two wheat varieties spaceflight carried were analyzed by SDS-PAGE. The quality score of Glu-1 of each site was calculated according to the quality rating system. The results showed that the sace flight carried could result in a higher frequency of HMW-GS gene mutation. The variance frequency of HMW-GS in SP3 of Shaan253 and Xinong1043 were 27.08% and 27.45%, and the quality score in SP3 of Shaan253 and Xinong1043 were 7 and 6, respectively. Shaan253 SP3 generation mutants were considered as high-quality wheat.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第2期193-196,共4页
Journal of Nuclear Agricultural Sciences
基金
农业部农业公益性行业科研专项(200803034)
西北农林科技大学校重点科研项目(2005)
