广西藤茶总黄酮对免疫抑制小鼠细胞免疫功能的影响

Effect of Total Flavonoids of Ampelopsis Grossedentata (Hand-Mazz) W.T.Wang on Cell-mediated Immunity of Immunosuppressed Mice

目的:探讨藤茶总黄酮(TF)对免疫抑制小鼠细胞免疫功能的影响。方法:以皮下注射地塞米松或环磷酰胺(CTX)复制小鼠免疫抑制模型,药物组给予不同剂量的TF(200,100和50mg·kg-1),并设空白组与模型对照组。分别以5,10μg·mL-1刀豆蛋白(ConA)刺激脾淋巴细胞,以MTT法测定其增殖功能;以MTT法和廓清率测定小鼠的NK细胞杀伤活性和单核细胞吞噬功能。结果:TF能够显著提高免疫抑制小鼠NK细胞杀伤活性和单核细胞廓清率。当ConA浓度为10μg·mL-1时,200mg·kg-1和100mg·kg-1TF能促进免疫抑制小鼠脾淋巴细胞增殖;当ConA浓度为5μg·mL-1时,200mg·kg-1TF能显著促进免疫抑制小鼠脾淋巴细胞增殖。结论:TF可提高免疫抑制小鼠的细胞免疫功能。

Objective： To investigate the immunomodulation of total flavonoids of ampelopsis grossedentata （Hand-Mazz） W.T. Wang （TF） on immunosuppressed mice. Methods： The immunodepressed mice were established by means of subcutaneous injection of dexamethasone or cyclophosphamide （CTX）. The mice of administration groups were given TF doses of 200, 100 and 50 mg·kg^-1 respectively in comparison with the control group and the model group. MTT method was used to detect lymphocyte proliferation induced by ConA （5, 10 μg·mL^-1）. The killing activity of NK cells and the phagocytosis of monocyte were tested by MTT method and clearance rate. Results： TF can significantly increase the killing activity of NK ceils and clearance index of monocyte in CTX-treated mice. 200 and 100 mg·kg^-1 dose of TF could enhance lymphocyte proliferation with ConA （10 μg·mL^-1）; 200 mg·kg^-1 dose of TF could significantly enhance lymphocyte proliferation with ConA （5μg·mL^-1）. Conclusion： TF can improve cell-mediated immunity of immunosuppressed mice.