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羽衣甘蓝花粉SCR_(13-b)基因的分离及其功能分析 被引量：4

Isolation and Functional Analysis of a S_(13-b)-locus Cysteine-rich Gene of Ornamental Kale (Brassica oleracea var.acephala)
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摘要 以羽衣甘蓝(Brassica oleracea var.acephala)S13-bS13-b自交不亲和系为试验材料,分离了控制花粉自交不亲和基因——S13-b单倍型富含半胱氨酸基因(SCR13-b),GenBank收录号为EF577028。SCR13-bcDNA序列含有237bp的开放读码框(ORF),编码一个含78个氨基酸的蛋白。SCR13-bgDNA含有一个758bp的内含子,内含子5′供体位点和3′受体位点边界序列符合GU-AG规则;DNA blot分析表明SCR13-b在基因组中只有单一拷贝;授粉生物活性分析试验显示原核表达SCR13-b融合蛋白能够启动SI反应,抑制杂交亲和花粉的萌发和生长。 The S13-b-IOcHS cysteine-rich gene （SCR13_b） was isolated from pollen of ornamental kale （Brassica oleracea var. acephala） S13-b homozygotes, GenBank accession number is EF577028. The SCR13-b cDNA sequence had an ORF of 237 bp, encoding a 78-aa protein. The genomic SCR13_b sequence contained a 758 bp intron with boundary sequences of GT at 5＇ site and AG at 3＇ site, which complied with the typical ‘ GT-AG rule’ in plants. Genomic DNA blot analysis showed that SCR13-b was a single copy gene in S13-bS13-b lines. Pollination bioassay showed that prokaryotic expressed recombinant SCR13-b can induce SI response and inhibit cross pollen germination and growth.
作者 蓝兴国 杨佳 李玉花
机构地区 东北林业大学生命科学学院
出处 《园艺学报》 CAS CSCD 北大核心 2009年第4期539-544,共6页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(30371189)
关键词 羽衣甘蓝 自交不亲和 原核表达 Brassica oleracea var. acephala self-incompatibility prokaryotic expression
分类号 S681.9 [农业科学—观赏园艺] Q781 [生物学—分子生物学]
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参考文献14

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二级参考文献73

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共引文献9

同被引文献80

  • 1郑斌,詹希美.信号肽序列及其在蛋白质表达中的应用[J].生物技术通讯,2005,16(3):296-298. 被引量：45
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  • 3于晓敏,蓝兴国,李玉花.泛素/26S蛋白酶体途径与显花植物自交不亲和反应[J].植物学通报,2006,23(2):197-206. 被引量：21
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引证文献4

二级引证文献10

园艺学报
2009年 第4期

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