瑞典常春藤漆斑病病原鉴定

Identification of the Pathogen Causing Black Spot Disease of Little Spurflower

通过病原菌的形态特征、培养性状和致病性测定的方法对引起瑞典常春藤漆斑病的病原菌进行了分离鉴定,采用PCR技术扩增病菌rDNA-ITS基因,获得一长度为523bp的DNA片段,序列测定结果表明该片段序列与GenBank已有的露湿漆斑菌序列的同源性达到了99%。综合两种方法鉴定结果,露湿漆斑菌是引起瑞典常春藤漆斑病的致病菌,露湿漆斑菌为该寄主上的真菌新病害。

A pathogenic fungus causing leaf spot of Plectranthus parviflorus was isolated and characterized based on the morphological features, cultural characteristics and pathogenicity. By using polymerase chain reaction （PCR） technique to amplify rDNA-ITS of the isolate, we obtained a 523 bp DNA fragment, the result showed that rDNA-ITS sequences of the isolate and Myrothecium roridum shared 99% homology, which was compared in National Center for Biotechnology Information （NCBI） GenBank. Synthesized two identification results indicated that Myrothecium roridurn was the pathogen causing black spot disease of Plectranthus parviflorus. This is the first report of Myrothecium leaf spot caused by Myrothecium roridum on Plectranthus parviflorus.