三叶因子1与胃癌发生发展过程中血管生成的关系

Expression of trefoil factor 1 in gastric cancer and its correlation with neovascularization

目的:观察三叶因子1(TFF1)和血管内皮生长因子(VEGF)在正常胃黏膜组织、癌旁组织和胃癌组织中的表达,并探讨TFF1与胃癌发生发展过程中血管生成的关系.方法:采用SP免疫组化方法检测42例正常组织、66例癌旁组织和66例胃癌组织中TFF1和VEGF的表达,以CD34标记血管内皮,测定MVD.结果:TFF1表达在正常胃黏膜组织→癌旁组织→胃癌组织中呈逐渐减弱趋势(209.40±16.00,199.12±16.68,189.17±16.20),差异具有显著性(P<0.01),而VEGF的表达和MVD呈逐渐上升趋势(69.7%,40.9%,35.7%;38.90±6.74,28.68±5.08,25.13±4.46).MVD值与TFF1灰度值呈正相关(r=0.811,P<0.01),即MVD值与TFF1的表达呈负相关.结论:TFF1作为一种胃癌特异性抑制因子在胃癌发生发展过程中并未促进新生血管的生成.

AIM： To investigate the expression of trefoil factor 1 （TFF1） and vascular endothelial growth factor （VEGF） in normal gastric mucosa, adjacent carcinoma and gastric carcinoma and to explore its role in neovas- cularization. METHODS： The expressions of TFF1, VEGF and MVD （CD34 monoclonal antibody label- ing） were determined by immunohistochemical method in 174 gastric specimens including 42 normal gastric mucosa, 66 adjacent carcinoma and 66 gastric carcinomas.RESULTS： In normal control group, adjacent carcinoma and gastric carcinoma group, the expression of TFF1 had a decreasing tendency （209.40 ± 16.00, 199.12 ± 16.68, 189.17 ± 16.20, P 〈 0.01）, but the expression of VEGF and the MVD had a increasing tendency （69.7%, 40.9%, 35.7%; 38.90 ±6.74, 28.68 ± 5.08, 25.13 ± 4.46）. MVD was positively correlated with grey levels of TFF1 （r = 0.811, P 〈 0.01）, in other words, there was a negative correlation between the MVD values and the expression of TFF1. CONCLUSION： TFF1, a specific anti-oncogene for gastric carcinoma, doesn＇t have a close correlation with tumor vessel neogenesis.