白细胞介素2促进宫颈癌细胞系HeLaS3免疫球蛋白G的表达

Interleukin 2 promotes the expression of immunoglobulin G in a cervical cancer cell line HeLaS3

目的:探讨白细胞介素2(interleukin2,IL-2)对宫颈癌细胞系HeLaS3表达免疫球蛋白G(immunoglobulin G,IgG)的影响。方法:用免疫荧光-流式细胞术(flow cytometry,FCM)检测IL-2刺激后HeLaS3胞膜和胞内IgG表达变化;用Western blot检测IL-2刺激HeLaS3后IgG的表达变化;用同时能扩增IgG四个亚类的引物,以RT-PCR法,从mRNA水平检测IL-2刺激后,HeLaS3γ链转录水平的变化。结果:免疫荧光-FCM结果显示,IL-2刺激48h后HeLaS3胞膜和胞内IgG表达明显上调;Western blot结果显示IL-2刺激48h后HeLaS3胞内IgG表达水平明显上调;RT-PCR显示IL-2刺激48h后HeLaS3γ链转录水平有所上升。结论:IL-2能促进HeLaS3IgG的表达。

Objective：To analyze the effect of interleukin 2 （IL-2） on immunoglobulin G （IgG） expression in cervical cancer cell line HeLaS3. Methods： By immunofluorescence-flow cytometry（FCM） , we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation. By western blot, we detected IgG expression variation of HeLaS3 after IL-2 stimulation. Meanwhile, by RT- PCR with primers designed to amplify four subclasses of IgG, we detected γ transcript variation in HeLaS3 after IL-2 stimulation at mRNA level. Results： The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours, membrane and cytoplasmic IgG expression elevated in HeLaS3. The result of Western blot showed after IL-2 stimulation for 48 hours, IgG expression elevated in HeLaS3. Meanwhile, the result of RT-PCR showed that T1 transcript mildly elevated after IL-2 stimulation for 48 hours. Conclusion： IL-2 can promote IgG expression in cervical cancer cell line HeLaS3.