人羊膜匀浆上清液的体外抗菌特性研究

Antimicrobial properties of human amniotic homogenate supernatant in vitro

目的研究人羊膜匀浆上清液的体外抗菌特性。方法取新鲜人羊膜制备匀浆上清液及"去上皮"羊膜,琼脂扩散法研究其抗菌作用及抗菌谱,微量肉汤稀释法比较其与青霉素等10种眼科临床常用抗生素的抗菌作用强弱,研究pH值、温度、时间对抗菌活性的影响,透射电镜观察其抗菌作用的可能靶点。结果羊膜中含有抗菌成分,存在于羊膜上皮细胞中;羊膜匀浆上清液抗菌谱广,其抑菌活性强于氯霉素、磺胺和头孢呋辛钠,与克林霉素和妥布霉素相当,杀菌活性不低于所选的10种抗生素;其性质稳定;抗菌作用靶点可能为病原微生物质膜。结论羊膜匀浆上清液有望成为治疗眼部感染的一种方便有效的方法,羊膜移植用于眼表重建时应采用带有完整上皮层的新鲜羊膜。

Objective To investigate the antimicrobial properties of human amniotic homogenate superna- rant （HAHS） in order to found a theoretical base for the use of human amniotic membrane in ophthalmological field. Methods Fresh human amniotic membranes were used to make HAHS and aeellular amniotic membranes. Then, we observed their antimierobial effects and antimicrobial spectrums, compared the antimicrobial capacity with 10 commonly used antibiotics in eyes, and investigated the effects of time, temperature and pH value on the antimicrobial capacity. Finally, transmission electron microscopy （TEM） was used to explore the possible targeting site of the antibiosis. Results Human amnion membrane contained antimicrobial components locating in its epithelial cells. HAHS had a broad antimicrohial spectrum and was steady in nature. Its antimicrobial capacity was stronger than those of sulfasulfonamide, chloromycetin and cefuroxime sodium. TEM indicated that the antimicrobial effect were exerted through plasma membrane of microorganism. Conclusion HAHS can be an effective and convenient treatment for ocular surface infectious diseases. Traditional amnion transplantation should employ fresh human amniotic membranes containing complete epithelial lamina to recon- struct the ocular surface.