氧化苦参碱对致敏小鼠气道高反应性和炎症的影响

The effects of oxymatrine on allergen-induced airway hyperresponsiveness and inflammation in mice

目的:观察氧化苦参碱对致敏小鼠气道高反应性和炎症的影响。方法:采用卵白蛋白致敏小鼠,反复攻击后形成小鼠哮喘模型。静脉注射乙酰甲胆碱(Mch)激发哮喘小鼠的气道高反应性,测定肺阻力(RL)和动态肺顺应性(Cdyn)。用支气管肺灌洗液(BALF)检测气道炎症细胞的浸润。结果:哮喘模型组与正常组小鼠比较,气道反应性明显增高,BALF中白细胞总数和嗜酸性粒细胞数目明显增多。氧化苦参碱50?150?450mg/kg灌胃给药呈剂量依赖抑制小鼠的气道高反应性和炎症反应。结论:氧化苦参碱能防治小鼠支气管哮喘,为氧化苦参碱运用于临床提供实验依据。

Objective： We evaluated the effect of oxymatine on allergen-induced airway hyperresponsiveness （AHR） and inflammation in mice. Methods： Sensitized mice were challenged with aerosolized ovalbumin for 7 days, oxymatine （50, 150 and 450mg/kg） and dexamethasone （ DXM 2mg/kg） were orally administered once daily during the period of challenge. AHR to methacholine （Mch） was determined by whole- body plethysmography. We measured the airway resistance and dynamic compliance of mice and evaluated the inflammationory cells in BALF. Results ： The model mice had significantly greater AHR than the control mice, development of AHR and the inflammation recreation was dosage dependently attenuated in mice that received oxymatine treatment. The inflammationory cells in BALF were significantly increased in model mice compared with control mice. Conclusion： these results suggest that oxymatine represents a potential new drug for the treatment of asthma.