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医院感染阴沟肠杆菌中整合子的检测及特点 被引量:3

Detection and characterization of class I integrons among Enterobacter cloacae isolates from inpatients
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摘要 目的研究整合子在医院感染阴沟肠杆菌中的分布及其与细菌耐药的相关性,探讨Ⅰ类整合子与临床耐药播散的关系。方法运用K-B法检测临床株的耐药表型,双纸片扩散法检测ESBLs,PCR扩增筛选含整合子的临床菌株,接合传递实验、套式PCR、质粒谱分析及DNA测序研究携带耐药基因的Ⅰ类整合子与耐药播散的关系。结果医院感染阴沟肠杆菌69.2%含Ⅰ类整合子,未检测出Ⅱ类和Ⅲ类整合子。Ⅰ类整合子可变区扩增片段大小从1.0~2.3kb不等,编码对氨基糖苷类抗生素、磺胺类药物和氯霉素耐药的基因。整合子阳性组中ESBLs、多重耐药菌均明显高于阴性组。整合子可通过质粒在不同菌属间水平传播。结论Ⅰ类整合子在医院感染阴沟肠杆菌中广泛分布,可通过质粒在不同菌属间水平传播,在耐药基因传播中起重要作用,应引起临床足够的重视。 Objectives Thirty nine unrelated clinical strains of Enterobacter cloacae collected from inpatients of the First Affiliated Hospital of Wenzhou Medical College. Methods Drug resistance test was performed by KB method. ESBL-positive strains were detected by double-disk synergy test. Integron was determined by PCR assay with integron-specific-primer. Conjugative transfer test, plasmid profile analysis, nested-PCR, and DNA sequence analysis were used to research for resistant horizontal transferable mechanism of integron mediated. Results Class Ⅰ integrons were detected in 27 of 39 (69. 2% ) unrelated clinical strains of E. cloacae from inpatients in Wenzhou. The presence of integrons was significantly correlated with simultaneous resistance to several antibiotics. Class Ⅰ integrons harbored four different gene cassette arrays. The size of amplicons was from 1,000 to 2, 300bp. Gene cassettes included genes encoding resistance to aminoglycosides (aadA1, aacIA2, aadA5, aacA4), trimethoprim-sulfamethoxazole ( dfrA12, dfrA17), and chloramphenicol (catB8). Strains positive for class I integron were highly related to muhidrug resistance and ESBLs. Class Ⅰ integron could horizontal transfer along with plasmid among bacteria. Conclusions Our results generally demonstrated the wide distribution of integron structures in E. cloacae isolates within the hospital environment throughout Wenzhou, China. Drug resistance were more liable to horizontal transfer via class Ⅰ integron along with plasmid. It implied the necessary for surveillance horizontal transference of antibiotic resistance gene among bacteria genus.
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2009年第4期254-256,I0001,I0002,共5页 Chinese Journal of Antibiotics
关键词 阴沟肠杆菌 整合子 水平播散 Enterobacter cloacae Integron Horizontal transfer
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参考文献17

  • 1Rowe-Magnus D A, GueroutA M, Mazel D. Bacterial resistance evolution by recruitment of super-integron gene cassettes [J]. Mol Microbiol, 2002, 43(6): 1657 - 1669.
  • 2Sobhan N, John J M , Charles H, et al. Gram-positive bacteria are a major reservoir of class 1 antibiotic resistance integrons in poultry litter[J]. PNAS, 2004, 101 (18): 7118 - 7122.
  • 3Pitout J D, Moland E S, Sanders C C, et al. Beta-lactamases and detection of β-lattain resistance in Enterobacter spp[J]. Antimicrob Agents Chemother, 1997, 41 ( 1 ): 35 - 39.
  • 4Levesque C, Piche L, Larose C, et al. PCR mapping of integrons reveals several novel combinations of resistance genes[J]. Antimicrob Agents Chemother, 1995, 39(1): 185- 191.
  • 5Lorian V. Antibiotics in laboratory medicine[M] .4th ed. Maryland USA: Maple Press, 1996:483 - 484.
  • 6Hall M A, Blok H E, Donders A R, et al. Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin[J]. J Infect Dis, 2003, 187(2): 251-259.
  • 7Fonseca E L, Vieira V V, Cipriano R, et al. Class 1 integrons in Pseudomonas aeruginosa isolates from clinical settings in Amazon region, Brazil[J]. FEMS Immunol Med Microbiol, 2005, 44(3): 303 - 309.
  • 8Turton J F, Kaufmann M E, Glover J, et al. Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom[J]. J Clin Microbiol, 2005, 43(7): 3074 - 3082.
  • 9Martinez-Freijo P, Fluit A C, Schmitz F J, et al. Class Ⅰ integrons in gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds[J]. J Antimicrob Chemother, 1998,42(6):689 -696.
  • 10Jones M E, Peters E, Weersink A M, et al. Widespread occurrence of integrons causing multiple antibiotic resistance in bacteria[J]. Lancet, 1997, 349(9067): 1742 - 1743.

二级参考文献13

  • 1魏全珍,张惠珍,钟馥霞,潘兢,钟定.超广谱β-内酰胺酶细菌感染监测及危险因素的临床研究[J].中华医院感染学杂志,2004,14(3):334-336. 被引量:24
  • 2张宏梅,石磊,李琳,陈清,陈义忠,张喜梅.沙门菌中第一类整合子的鉴定及特性分析[J].中华微生物学和免疫学杂志,2004,24(3):214-217. 被引量:39
  • 3National Committee for Clinical Laboratory Standards.Performance standards for antimicrobial disk susceptibility tests[S].6th ed.Approved standard M2-A6(M100-S7).Wayne:NCCLS,2003.
  • 4WeinsteinRA.产β—内酰胺酶耐药菌—全球性难题[J].急救快车,2002,8:18-18.
  • 5Chanawong A,Xiong J,Hawkey PM,et al.Three cefotaximases,CTX-M-9,CTX-M-13,CTX-M-14,among Enterobacteriaceae in the People's Republic of China[J].Antimicrob Agents Chemother,2002,46(3):630-637.
  • 6Rosser S J, Young H K. Identification and characterization of class 1 integrons in bacteria from an aquatic environment[J]. J Antimicrob Chemother,1999,44(1) : 11.
  • 7Mazel D, Dychinco B, Webb V A, et al. Antibiotic resistance in the ECOR collection: integrons and identification of a novel and gene[J]. Antimicrob Agents Chemother,2000,44(6):1568.
  • 8Hall R M, Collis C M. Mobile gene cassettes and integrons : capture and spread of genes by site-specific recombination[J]. Mol Microbiol, 1995,15 (4) : 593.
  • 9Rowe-Magnus D A, Guerout A M, Mazel D. Bacterial resistance evolution by recruitment of super-integron gene cassettes[J]. Mol Microbiol,2002,43(6):1657.
  • 10陈民钧.细菌对β-内酰胺药的耐药性及检测方法[J].中华检验医学杂志,2001,24(4):197-200. 被引量:161

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