脑星形细胞肿瘤中Survivin、PTEN蛋白表达与细胞凋亡的相关性

Expression of Survivin, PTEN and its relationship with cell apoptosis in brain astrocytomas

目的探讨脑星形细胞肿瘤中细胞凋亡的变化及其相关基因Survivin、PTEN的表达和二者的相关性。方法应用TUNEL法检测细胞凋亡，免疫组织化学SP法检测Survivin、PTEN、Ki-67蛋白表达。结果在73例脑星形细胞肿瘤中Survivin的阳性表达率为64．4％，加权记分和凋亡指数（AI）之间呈明显负相关（r=-0．85，P〈0．01），Survivin阳性组AI较Survivin阴性组降低（P=0．035）。PTEN的阳性表达率为72．6％，加权记分和AI之间呈正相关（r=0．303，P=0．026），PTEN阳性组AI较PTEN阴性组增高（P=0．034）。AI与肿瘤的病理分级呈显著负相关（r=-0．423，P=0．001），增生指数（PI）则随着肿瘤的级别增高而明显增高（r=0．729，P〈0．01）。结论脑星形细胞肿瘤中，细胞凋亡受多种因素的影响，AI的高低与Survivin、PTEN的表达均有关。Survivin高表达，AI值下降；PTEN高表达，AI值则增高。

Objective To investigate the cell apoptosis and the expression level of its related gene Survivin, PTEN. Methods Apoptotic cells and bodies were detected by TUNEL. Immunohistochemical stains were performed to examine the expression of Survivin, PTEN and Ki-67. The software package SPSS 10.0 was used for statistical analysis. Results The Survivin positive rate in 73 tumors was 64.4 %, and there was significant negative correlation between Survivin weighted score and AI （r =-0.85,P 〈0.01 ）. The mean AI of survivin-positive tumors was lower than that of Survivin-negative tumors（P =0.035）. The PTEN positive rate in 73 tumors was 72.6 %, there was positive correlation between PTEN weighted score and AI （P =0.026）. The mean AI of PTEN-positive tumors was higher than that of PTEN-negative tumors （P =0.034）. There was significant negative correlation between AI and pathological grade and positive correlation between PI and pathological grade（r =-0.432, P =0.001, r =0.729, P 〈0.01）. Conclusion Cell apoptosis was affected by some factors. AI was associated with the expression of Survivin and PTEN.