p27kip1、CyelinD1、CyelinE在膀胱移行细胞癌中表达的意义

Expression and significance of p27kip1, CyclinD1, CyclinE in transitional cell carcinoma of bladder

目的探讨p27kip1、CyclinE、CyclinD1在膀胱移行细胞癌（BTCC）中的表达与意义，为认识BTCC发生、发展机制提供线索。方法应用免疫组织化学SP法检测41例BTCC和12例膀胱炎中p27kip1、CyclinE、CyclinD1的表达。结果p27kip1在BTCC中阳性表达随临床分期和病理分级的增高而降低（P〈0．05），有淋巴转移组与无淋巴转移组比较差异无统计学意义（P〉O．05）。CyclinD1在不同BTCC临床分期和病理分级中的阳性表达差异无统计学意义（P〉0．05）。CyclinE在BTCC中阳性表达随临床分期和病理分级的增高而增高（P〈0．05），有淋巴转移组明显高于无淋巴转移组（P〈0．05）。结论p27kip1对BTCC的发生、发展可能起抑制作用，CyclinD1过表达可能在膀胱移行细胞癌发生的早期起作用，不参与膀胱移行细胞癌的浸润转移过程。CyclinE可能参与BTCC的发生、发展过程。

Objective To investigate the expression of p27kip1, CyclinE, CyclinD1 in bladder transitional cell carcinoma （BTCC）, and evaluate their relationship with tumour cell proliferation. Methods To study the expression of p27kip1, CyclinE and CyclinD1 protein in 41 cases of paraffin-embedded BTCC tissue and in 12 cases of cystitis tissues by immunohistochemical method SP. Results There was a negative relationship between p27kip1 expression and clinical stage and pathological grade.There was no relationship with lymph node metastasis （P 〉0.05）. There was a positive relationship between CyclinE expression and clinical stage, pathological grade and lymph node metastasis （P 〈0.05）. There was no relationship between CyclinD1 expression and clinical stage, pathological grade and lymph node metastasis （P 〉0.05）. Conclusion The results of this study showed that the abnormal expression of p27kip1, CyclinE, may play important roles in the genesis and progression of BTCC. CyclinD1 may take activation in the early term of BTCC.