长白山百合的离体快繁技术研究

Research on Rapid Propagation Technique of Lily in Changbai Mounatain

为明确长白山百合离体组织培养及植株的再生能力,对长白山百合不同部位的组织进行了离体培养,并进行了不同培养激素的对比试验。结果表明:长白山百合外中部鳞片小鳞茎形成率明显高于内部,再生小鳞茎的出现部位主要位于鳞片基部;在附加不同激素水平及其配比的MS培养基上培养,长白山百合以MS(植物组织培养基本培养基)+0.5mg/LBA(细胞分裂素)+0.1mg/LNAA(萘乙酸)+3%蔗糖和MS+0.5mg/LBA+0.5mg/LNAA+3%蔗糖等2个培养基生长最好。

The purpose of this experiment was to study Lily vitro tissue and plant regeneration, for a small lily bulb in vitro production of providing a scientific basis. This passage studied the impaction small scales from the bulb occurred and formed small plantlets bulb factors and the same species, different parts of the bulb formation of the small bulbs of different capacities. This test proved that foreign central flake small bulb formation rate was higher than internal, the renewable small lily bulbs the emergence mainly lie in the basis of scales. Training different hormone comparative testing, the final screening of Changbai Mountain Lily was suitable for the tissue culture medium. Added with different hormone levels and the ratio of MS medium culture, Changbai Mountain Lily will grow prosperously with the following formula： MS （plant tissue culture medium） ＋0. 5 mg/L BA （CTK） ＋0. 1 mg/L NAA （NAA） ＋3% sucrose and MS＋0. 5 mg/L BA＋0. 5 mg/L NAA＋3% sucrose medium.