CD4^＋CD25%＋FoxP3^＋调节性T细胞在肺癌患者体内检测及其意义

Significance of measurement of CD4^＋CD25%＋FoxP3^＋ egulatory T cell in patients with lung cancer

目的研究肺癌患者外周血和肿瘤组织CD4^＋CD25^＋FoxP3^＋调节性T细胞（Treg）的异常聚集，探讨其在肺癌患者体内调节免疫抑制作用。方法采用流式细胞术对28例肺癌患者的癌组织，相应的癌旁组织和外周血及15例正常志愿者外周血中CD4^＋CD25^＋FoxP3^＋调节性T细胞的检测。结果肺癌患者癌组织中CD4^＋CD25^＋FoxP3^＋调节性T细胞在CD4^＋T细胞中所占比例是（10．5±1．6）％；外周血中和癌旁组织表达分别是（5．2±2．1）％和（3．6±1．2）％，癌组织明显高于外周血和癌旁组织（P〈0．05）。肺癌患者外周血中Treg细胞（5．2±2．1）％明显高于志愿者（1．8±1．2）％。结论CD4^＋CD25^＋FoxP3^＋调节性T细胞在肺癌组织及外周血中高表达，促进了肿瘤的进一步恶化，在癌组织学分型上也存在明显差异，肺腺癌表达阳性较高，可以作为肺癌免疫治疗的靶点。

Objective To study the peripheral blood of patients with lung cancer and tumor tissue CD4^＋CD25^＋FoxP3^＋ regulatory T cells （Treg）- of abnormal accumulation and explore its regulation in pa- tients with lung cancer in the body immunity. Methods The percentage of the CD4^＋CD25^＋FoxP3^＋ regulatory T cells in the tumor tissues, the adjacent tissues and peripheral blood from 28 patients with NSCLC and 15 healthy controls was detected by flow cytometry. Results The proportion of CD4^＋CD25^＋FoxP3^＋ regulatory T cells in CD4^＋ T cells was （ 10.5 ＋ 1.1 ） % in the tumor tissues; the percent of CD4^＋CD25^＋FoxP3^＋ regulatory T lymphocytes in peripheral blood and the normal tissues were （5.2 ± 2. 1 ） % and （3.6 ± 1.2） % in the 28 patients with lung cancer. The percent of the CD4^＋CD25^＋FoxP3^＋ regulatory T lymphocytes in the peripheral blood of patients with lung cancer （5.2±2.1 ） % was significantly higher than that of volunteers （ 1.8±1.2） %. Conclusions CD4^＋CD25^＋FoxP3^＋ regulatory T cells in the peripheral blood and lung cancer is high - expression and promote the further deterioration of the tumors.